Absence of biallelic TCRγ deletion predicts induction failure and poorer outcomes in childhood T-cell acute lymphoblastic leukemia

• Published in: Pediatric blood & cancer 2012-06, Vol.58 (6), p.846-851
• Main Authors: Yang, Yung-Li, Hsiao, Chih-Cheng, Chen, Hsuan-Yu, Lin, Kai-Hsin, Jou, Shiann-Tarng, Chen, Jiann-Shiuh, Chang, Te-Kau, Sheen, Jiunn-Ming, Yu, Sung-Liang, Lu, Meng-Yao, Cheng, Chao-Neng, Wu, Kang-Hsi, Wang, Shih-Chung, Wang, Jiaan-Der, Chang, Hsiu-Hao, Lin, Shu-Rung, Lin, Shu-Wha, Lin, Dong-Tsamn
• Format: Article
• Language: English
• Subjects: absence of TCRγ deletion; Antineoplastic Combined Chemotherapy Protocols - therapeutic use; childhood T-cell ALL; Disease-Free Survival; Gene Deletion; Genes, T-Cell Receptor gamma - genetics; Humans; Immunophenotyping; Induction Chemotherapy; Kaplan-Meier Estimate; Polymerase Chain Reaction; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma - drug therapy; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma - genetics; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma - mortality; Prognosis; Proportional Hazards Models; Real-Time Polymerase Chain Reaction; Receptors, Antigen, T-Cell, gamma-delta - genetics; Reverse Transcriptase Polymerase Chain Reaction; Treatment Outcome
• ISSN: 1545-5009; 1545-5017
• DOI: 10.1002/pbc.24021

Background The absence of biallelic TCRγ deletion (ABD) is a characteristic of early thymocyte precursors before V(D)J recombination. The ABD was reported to predict early treatment failure in T‐cell acute lymphoblastic leukemia (ALL). This study aimed to investigate its prognostic value in Taiwanese patients with T‐cell ALL. Procedure Forty‐five children with T‐cell ALL were enrolled from six medical centers in Taiwan. Quantitative DNA polymerase chain reaction (Q‐PCR) was performed to check the status of TCRγ deletion. The threshold for homozygous deletions by Q‐PCR was defined as a fold‐change