An immunochemical method for detection and analysis of changes in methylome

DNA methylation is an important epigenetic modification involved in the ability of an organism to respond to stress and adaptation. It has been implicated in development, differentiation, oncogenesis, chromatin remodelling, nutrigenomics, and appears to play a pivotal role in many regulatory and ada...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2012-02, Vol.56 (2), p.260-267
Main Authors: Deobagkar, Deepti D., Panikar, Chitra, Rajpathak, Shriram N., Shaiwale, Nayana S., Mukherjee, Sanjay
Format: Article
Language:English
Subjects:
Animals
Brain - enzymology
Cell Line
Cytosine - chemistry
DNA methyl transferase
DNA methylation
DNA Methylation - radiation effects
DNA Modification Methylases - analysis
DNA Modification Methylases - antagonists & inhibitors
DNA Modification Methylases - chemistry
Enzyme Activation
Enzyme Assays
Epigenesis, Genetic
Female
Genome
Immunochemistry - methods
Liver - enzymology
Methyl cytosine
Mice
Mice, Inbred BALB C
Microarray
Oligonucleotide Array Sequence Analysis - methods
Radiation
Sensitivity and Specificity
Stress and adaptation
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Summary:DNA methylation is an important epigenetic modification involved in the ability of an organism to respond to stress and adaptation. It has been implicated in development, differentiation, oncogenesis, chromatin remodelling, nutrigenomics, and appears to play a pivotal role in many regulatory and adaptive functions. It is therefore important to analyze the status of DNA methylation and its changes under various developmental, carcinogenic, pharmacological, and environmental conditions. In this report we describe an immunochemical method for the detection of genome wide DNA methylation and its alterations under various conditions along with the analysis of DNA methyltransferase activity. The ability of this approach to detect and provide a map of methylomic changes in a genome facilitates assessment of various agents and conditions which can alter this important epigenetic signal. This experimental system permits rapid evaluation of potential target genes which would be modulated by DNA methylation changes and thus the gene networks that govern the processes.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2011.10.003