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Serum protein N-glycosylation in paediatric non-alcoholic fatty liver disease
Summary Objective We have previously shown the potential of glycomics to distinguish patients with steatosis from patients with non‐alcoholic steatohepatitis (NASH) in an adult population. The pattern of disease in paediatric patients is distinct from adults. The objective of this study was to chara...
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| Published in: | Pediatric obesity 2012-04, Vol.7 (2), p.165-173 |
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| container_issue | 2 |
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| container_title | Pediatric obesity |
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| creator | Blomme, B. Fitzpatrick, E. Quaglia, A. De Bruyne, R. Dhawan, A. Van Vlierberghe, H. |
| description | Summary
Objective
We have previously shown the potential of glycomics to distinguish patients with steatosis from patients with non‐alcoholic steatohepatitis (NASH) in an adult population. The pattern of disease in paediatric patients is distinct from adults. The objective of this study was to characterize the N
‐glycomic profile of children with varying degrees of non‐alcoholic fatty liver disease (NAFLD) and identify potential biomarker profiles of disease.
Methods
Serum protein N‐glycosylation patterns of 51 paediatric NAFLD patients were assessed with deoxyribonucleic acid sequencer‐assisted fluorophore‐assisted capillary electrophoresis and compared with histology.
Results
Peak 1 (NGA2F) is the most significantly elevated N‐glycan in paediatric NASH patients with peak 5 (NA2) demonstrating the largest decrease. The logarithmically transformed ratio of peak 1 to peak 5 was −0.85 (standard deviation [SD] 0.22) in patients with steatosis and borderline NASH and −0.73 (SD 0.12) in NASH (P = 0.02). The biomarker correlated well with the amount of lobular inflammation with a consistent increase of marker score in ascending stage of lobular inflammation. There was also a trend in differentiating patients with significant fibrosis ≥F2; −0.74 (SD 0.13) from patients with no/minimal fibrosis |
| doi_str_mv | 10.1111/j.2047-6310.2011.00024.x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_934257977</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>934257977</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4554-d3ba12b16b58baf51070f1ec296f2ed902827cdaf2a9fe4c3b8af507b06128f3</originalsourceid><addsrcrecordid>eNqNkF1PwyAUhonRqJn7C6Z3XnUCpaVNvDFG58f82pZ4SSg9KJO1Ezpd_73M6a7lBs7heQ_kQSgieEDCOp0NKGY8zpLQoJiQAcaYssFqBx1uL3a3Z0wPUN_7WYBwhkmG2T46oJQljKf8EN1PwC3n0cI1LZg6eohfbaca31nZmqaOQmshoTKydUZFdVPH0qrmrbGh0rJtu8iaT3BRZTxID0doT0vrof-799D06nJ6cR2PHoc3F-ejWLE0ZXGVlJLQkmRlmpdSpwRzrAkoWmSaQlVgmlOuKqmpLDQwlZR5oDAvcUZorpMeOtmMDd_-WIJvxdx4BdbKGpqlF0XCaMoLzgOZb0jlGu8daLFwZi5dJwgWa5tiJtaixFqaWNsUPzbFKkSPfx9ZlnOotsE_dwE42wBfxkL378Hi5vbpMczooXgTN76F1TYu3bvIeMJT8fIwFOPxdPQ8ebkT4-Qb9dGSuw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>934257977</pqid></control><display><type>article</type><title>Serum protein N-glycosylation in paediatric non-alcoholic fatty liver disease</title><source>Wiley</source><creator>Blomme, B. ; Fitzpatrick, E. ; Quaglia, A. ; De Bruyne, R. ; Dhawan, A. ; Van Vlierberghe, H.</creator><creatorcontrib>Blomme, B. ; Fitzpatrick, E. ; Quaglia, A. ; De Bruyne, R. ; Dhawan, A. ; Van Vlierberghe, H.</creatorcontrib><description>Summary
Objective
We have previously shown the potential of glycomics to distinguish patients with steatosis from patients with non‐alcoholic steatohepatitis (NASH) in an adult population. The pattern of disease in paediatric patients is distinct from adults. The objective of this study was to characterize the N
‐glycomic profile of children with varying degrees of non‐alcoholic fatty liver disease (NAFLD) and identify potential biomarker profiles of disease.
Methods
Serum protein N‐glycosylation patterns of 51 paediatric NAFLD patients were assessed with deoxyribonucleic acid sequencer‐assisted fluorophore‐assisted capillary electrophoresis and compared with histology.
Results
Peak 1 (NGA2F) is the most significantly elevated N‐glycan in paediatric NASH patients with peak 5 (NA2) demonstrating the largest decrease. The logarithmically transformed ratio of peak 1 to peak 5 was −0.85 (standard deviation [SD] 0.22) in patients with steatosis and borderline NASH and −0.73 (SD 0.12) in NASH (P = 0.02). The biomarker correlated well with the amount of lobular inflammation with a consistent increase of marker score in ascending stage of lobular inflammation. There was also a trend in differentiating patients with significant fibrosis ≥F2; −0.74 (SD 0.13) from patients with no/minimal fibrosis <F2; −0.86 (SD 0.24), P = 0.06. Analysis of the N‐glycans on immunoglobulin G confirmed the undergalactosylation status typical for chronic inflammatory conditions.
Conclusions
This study is the first glycomic analysis performed in a paediatric NAFLD population. In agreement with the results obtained in adults, B cells play a dominant role in the N‐glycan alterations of paediatric NASH patients.</description><identifier>ISSN: 2047-6302</identifier><identifier>EISSN: 2047-6310</identifier><identifier>DOI: 10.1111/j.2047-6310.2011.00024.x</identifier><identifier>PMID: 22434757</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Biomarkers - blood ; Biopsy ; Blood Proteins - metabolism ; Child ; Cohort Studies ; Diagnosis, Differential ; Fatty Liver - diagnosis ; Fatty Liver - metabolism ; Fatty Liver - pathology ; Female ; glycomics ; Glycomics - methods ; Glycosylation ; Humans ; immunoglobulin G ; Immunoglobulin G - blood ; Liver - metabolism ; Liver - pathology ; liver steatosis ; Male ; Non-alcoholic Fatty Liver Disease ; protein glycosylation</subject><ispartof>Pediatric obesity, 2012-04, Vol.7 (2), p.165-173</ispartof><rights>2012 The Authors. Pediatric Obesity © 2012 International Association for the Study of Obesity</rights><rights>2012 The Authors. Pediatric Obesity © 2012 International Association for the Study of Obesity.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4554-d3ba12b16b58baf51070f1ec296f2ed902827cdaf2a9fe4c3b8af507b06128f3</citedby><cites>FETCH-LOGICAL-c4554-d3ba12b16b58baf51070f1ec296f2ed902827cdaf2a9fe4c3b8af507b06128f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22434757$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blomme, B.</creatorcontrib><creatorcontrib>Fitzpatrick, E.</creatorcontrib><creatorcontrib>Quaglia, A.</creatorcontrib><creatorcontrib>De Bruyne, R.</creatorcontrib><creatorcontrib>Dhawan, A.</creatorcontrib><creatorcontrib>Van Vlierberghe, H.</creatorcontrib><title>Serum protein N-glycosylation in paediatric non-alcoholic fatty liver disease</title><title>Pediatric obesity</title><addtitle>Pediatric Obesity</addtitle><description>Summary
Objective
We have previously shown the potential of glycomics to distinguish patients with steatosis from patients with non‐alcoholic steatohepatitis (NASH) in an adult population. The pattern of disease in paediatric patients is distinct from adults. The objective of this study was to characterize the N
‐glycomic profile of children with varying degrees of non‐alcoholic fatty liver disease (NAFLD) and identify potential biomarker profiles of disease.
Methods
Serum protein N‐glycosylation patterns of 51 paediatric NAFLD patients were assessed with deoxyribonucleic acid sequencer‐assisted fluorophore‐assisted capillary electrophoresis and compared with histology.
Results
Peak 1 (NGA2F) is the most significantly elevated N‐glycan in paediatric NASH patients with peak 5 (NA2) demonstrating the largest decrease. The logarithmically transformed ratio of peak 1 to peak 5 was −0.85 (standard deviation [SD] 0.22) in patients with steatosis and borderline NASH and −0.73 (SD 0.12) in NASH (P = 0.02). The biomarker correlated well with the amount of lobular inflammation with a consistent increase of marker score in ascending stage of lobular inflammation. There was also a trend in differentiating patients with significant fibrosis ≥F2; −0.74 (SD 0.13) from patients with no/minimal fibrosis <F2; −0.86 (SD 0.24), P = 0.06. Analysis of the N‐glycans on immunoglobulin G confirmed the undergalactosylation status typical for chronic inflammatory conditions.
Conclusions
This study is the first glycomic analysis performed in a paediatric NAFLD population. In agreement with the results obtained in adults, B cells play a dominant role in the N‐glycan alterations of paediatric NASH patients.</description><subject>Adolescent</subject><subject>Biomarkers - blood</subject><subject>Biopsy</subject><subject>Blood Proteins - metabolism</subject><subject>Child</subject><subject>Cohort Studies</subject><subject>Diagnosis, Differential</subject><subject>Fatty Liver - diagnosis</subject><subject>Fatty Liver - metabolism</subject><subject>Fatty Liver - pathology</subject><subject>Female</subject><subject>glycomics</subject><subject>Glycomics - methods</subject><subject>Glycosylation</subject><subject>Humans</subject><subject>immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>liver steatosis</subject><subject>Male</subject><subject>Non-alcoholic Fatty Liver Disease</subject><subject>protein glycosylation</subject><issn>2047-6302</issn><issn>2047-6310</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkF1PwyAUhonRqJn7C6Z3XnUCpaVNvDFG58f82pZ4SSg9KJO1Ezpd_73M6a7lBs7heQ_kQSgieEDCOp0NKGY8zpLQoJiQAcaYssFqBx1uL3a3Z0wPUN_7WYBwhkmG2T46oJQljKf8EN1PwC3n0cI1LZg6eohfbaca31nZmqaOQmshoTKydUZFdVPH0qrmrbGh0rJtu8iaT3BRZTxID0doT0vrof-799D06nJ6cR2PHoc3F-ejWLE0ZXGVlJLQkmRlmpdSpwRzrAkoWmSaQlVgmlOuKqmpLDQwlZR5oDAvcUZorpMeOtmMDd_-WIJvxdx4BdbKGpqlF0XCaMoLzgOZb0jlGu8daLFwZi5dJwgWa5tiJtaixFqaWNsUPzbFKkSPfx9ZlnOotsE_dwE42wBfxkL378Hi5vbpMczooXgTN76F1TYu3bvIeMJT8fIwFOPxdPQ8ebkT4-Qb9dGSuw</recordid><startdate>201204</startdate><enddate>201204</enddate><creator>Blomme, B.</creator><creator>Fitzpatrick, E.</creator><creator>Quaglia, A.</creator><creator>De Bruyne, R.</creator><creator>Dhawan, A.</creator><creator>Van Vlierberghe, H.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201204</creationdate><title>Serum protein N-glycosylation in paediatric non-alcoholic fatty liver disease</title><author>Blomme, B. ; Fitzpatrick, E. ; Quaglia, A. ; De Bruyne, R. ; Dhawan, A. ; Van Vlierberghe, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4554-d3ba12b16b58baf51070f1ec296f2ed902827cdaf2a9fe4c3b8af507b06128f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adolescent</topic><topic>Biomarkers - blood</topic><topic>Biopsy</topic><topic>Blood Proteins - metabolism</topic><topic>Child</topic><topic>Cohort Studies</topic><topic>Diagnosis, Differential</topic><topic>Fatty Liver - diagnosis</topic><topic>Fatty Liver - metabolism</topic><topic>Fatty Liver - pathology</topic><topic>Female</topic><topic>glycomics</topic><topic>Glycomics - methods</topic><topic>Glycosylation</topic><topic>Humans</topic><topic>immunoglobulin G</topic><topic>Immunoglobulin G - blood</topic><topic>Liver - metabolism</topic><topic>Liver - pathology</topic><topic>liver steatosis</topic><topic>Male</topic><topic>Non-alcoholic Fatty Liver Disease</topic><topic>protein glycosylation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blomme, B.</creatorcontrib><creatorcontrib>Fitzpatrick, E.</creatorcontrib><creatorcontrib>Quaglia, A.</creatorcontrib><creatorcontrib>De Bruyne, R.</creatorcontrib><creatorcontrib>Dhawan, A.</creatorcontrib><creatorcontrib>Van Vlierberghe, H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatric obesity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blomme, B.</au><au>Fitzpatrick, E.</au><au>Quaglia, A.</au><au>De Bruyne, R.</au><au>Dhawan, A.</au><au>Van Vlierberghe, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum protein N-glycosylation in paediatric non-alcoholic fatty liver disease</atitle><jtitle>Pediatric obesity</jtitle><addtitle>Pediatric Obesity</addtitle><date>2012-04</date><risdate>2012</risdate><volume>7</volume><issue>2</issue><spage>165</spage><epage>173</epage><pages>165-173</pages><issn>2047-6302</issn><eissn>2047-6310</eissn><abstract>Summary
Objective
We have previously shown the potential of glycomics to distinguish patients with steatosis from patients with non‐alcoholic steatohepatitis (NASH) in an adult population. The pattern of disease in paediatric patients is distinct from adults. The objective of this study was to characterize the N
‐glycomic profile of children with varying degrees of non‐alcoholic fatty liver disease (NAFLD) and identify potential biomarker profiles of disease.
Methods
Serum protein N‐glycosylation patterns of 51 paediatric NAFLD patients were assessed with deoxyribonucleic acid sequencer‐assisted fluorophore‐assisted capillary electrophoresis and compared with histology.
Results
Peak 1 (NGA2F) is the most significantly elevated N‐glycan in paediatric NASH patients with peak 5 (NA2) demonstrating the largest decrease. The logarithmically transformed ratio of peak 1 to peak 5 was −0.85 (standard deviation [SD] 0.22) in patients with steatosis and borderline NASH and −0.73 (SD 0.12) in NASH (P = 0.02). The biomarker correlated well with the amount of lobular inflammation with a consistent increase of marker score in ascending stage of lobular inflammation. There was also a trend in differentiating patients with significant fibrosis ≥F2; −0.74 (SD 0.13) from patients with no/minimal fibrosis <F2; −0.86 (SD 0.24), P = 0.06. Analysis of the N‐glycans on immunoglobulin G confirmed the undergalactosylation status typical for chronic inflammatory conditions.
Conclusions
This study is the first glycomic analysis performed in a paediatric NAFLD population. In agreement with the results obtained in adults, B cells play a dominant role in the N‐glycan alterations of paediatric NASH patients.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>22434757</pmid><doi>10.1111/j.2047-6310.2011.00024.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Biomarkers - blood Biopsy Blood Proteins - metabolism Child Cohort Studies Diagnosis, Differential Fatty Liver - diagnosis Fatty Liver - metabolism Fatty Liver - pathology Female glycomics Glycomics - methods Glycosylation Humans immunoglobulin G Immunoglobulin G - blood Liver - metabolism Liver - pathology liver steatosis Male Non-alcoholic Fatty Liver Disease protein glycosylation |
| title | Serum protein N-glycosylation in paediatric non-alcoholic fatty liver disease |
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