Novel Cyanine Dyes with Vinylsulfone Group for Labeling Biomolecules

Novel vinylsulfone cyanine dyes (em. 550–850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and react...

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Bibliographic Details
Published in:Bioconjugate chemistry 2012-03, Vol.23 (3), p.350-362
Main Authors: Park, Jin Woo, Kim, YoungSoo, Lee, Kee-Jung, Kim, Dong Jin
Format: Article
Language:English
Subjects:
Aqueous solutions
Chemical reactions
Chromatography, Liquid
Coloring Agents - chemistry
Dyes
Fluorescence
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Molecules
Proteins - chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sulfones - chemistry
Temperature
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Summary:Novel vinylsulfone cyanine dyes (em. 550–850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc200232d