Genetic structure of Varroa destructor populations infesting Apis mellifera colonies in Argentina

• Published in: Experimental & applied acarology 2012-04, Vol.56 (4), p.309-318
• Main Authors: Maggi, M, Medici, S, Quintana, S, Ruffinengo, S, Marcángeli, J, Gimenez Martinez, P, Fuselli, S, Eguaras, M
• Format: Article
• Language: English
• Subjects: Animal Ecology; Animal Genetics and Genomics; Animal Systematics/Taxonomy/Biogeography; Animals; apiaries; Apis mellifera; Base Sequence; Bees - parasitology; Biomedical and Life Sciences; Colonies; Cosmopolites; DNA, Mitochondrial - chemistry; DNA, Mitochondrial - genetics; Electron Transport Complex IV - chemistry; Electron Transport Complex IV - genetics; Entomology; Female; Gene mapping; Genetic analysis; Genetic structure; Genetic Variation; Genotypes; Haplotypes; Life Sciences; Mites; Mitochondrial DNA; Molecular Sequence Data; Nucleotide sequence; nucleotide sequences; Polymerase Chain Reaction; Sampling; Sequence Alignment; Sequence Analysis, DNA; Varroa destructor; Varroidae - enzymology; Varroidae - genetics; worker honey bees; Workers
• ISSN: 0168-8162; 1572-9702
• DOI: 10.1007/s10493-012-9526-0

Although mitochondrial DNA mapping of Varroa destructor revealed the presence of several haplotypes, only two of them (Korean and Japanese haplotypes) were capable to infest Apis mellifera populations. Even though the Korean haplotype is the only one that has been reported in Argentina, these conclusions were based on mites sampled in apiaries from a specific geographical place (Buenos Aires province). To study mites from several sites of Argentina could reveal the presence of the Japanese genotype, especially considering sites near to Brazil, where Japanese haplotype was already detected. The aim of this work was to study the genetic structure of V. destructor populations from apiaries located in various provinces of Argentina, in order to determine the presence of different haplotypes. The study was carried out between January 2006 and December 2009. Phoretic adult Varroa mites were collected from honey bee workers sampled from colonies of A. mellifera located in Entre Ríos, Buenos Aires, Corrientes, Río Negro, Santa Cruz and Neuquén provinces. Twenty female mites from each sampling site were used to carry out the genetic analysis. For DNA extraction a nondestructive method was used. DNA sequences were compared to Korean haplotype (AF106899) and Japanese haplotype (AF106897). All DNA sequences obtained from mite populations sampled in Argentina, share 98% of similitude with Korean Haplotype (AF106899). Taking into account these results, we are able to conclude that Korean haplotype is cosmopolite in Argentina.