Plasma persistence of 2-aminothiazoline-4-carboxylic acid in rat system determined by liquid chromatography tandem mass spectrometry

► ATCA persistence was determined after injecting it directly to the blood stream. ► ATCA concentration in blood decreased to half within 2.5h in the rat system. ► After the initial loss, ATCA level stayed constant (700ng/ml) over 48h. ► Endogenous ATCA level in blood was found 141ng/ml. ► The role...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2012-04, Vol.891-892, p.81-84
Main Authors: Petrikovics, Ilona, Yu, Jorn C.C., Thompson, David E., Jayanna, Prashanth, Logue, Brian A., Nasr, Jessica, Bhandari, Raj K., Baskin, Steven I., Rockwood, Gary
Format: Article
Language:English
Subjects:
2-Aminothiazoline-4-carboxylic acid (ATCA)
Animals
biomarkers
Chromatography
Chromatography, Liquid - methods
Cyanide exposure
cyanides
Diagnostic biomarker
Diagnostic systems
Extraction
Forensic biomarker
forensic sciences
Imprinted polymers
intravenous injection
LC–MS/MS
liquid chromatography
Liquids
Male
Mass spectrometry
molecular imprinting
polymers
Rats
solid phase extraction
Solid phases
sorption
tandem mass spectrometry
Tandem Mass Spectrometry - methods
Thiazoles - blood
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Summary:► ATCA persistence was determined after injecting it directly to the blood stream. ► ATCA concentration in blood decreased to half within 2.5h in the rat system. ► After the initial loss, ATCA level stayed constant (700ng/ml) over 48h. ► Endogenous ATCA level in blood was found 141ng/ml. ► The role of ATCA as a biomarker for CN exposure needs more future investigations. 2-Aminothiazoline-4-carboxylic acid (ATCA) was intravenously injected to rats in order to investigate its plasma distribution. ATCA was extracted from plasma samples by solid phase extraction (SPE) and molecularly imprinted polymer stir bar sorption extraction (MIP-SBSE). Detection and quantification of ATCA were achieved by using liquid chromatography–tandem mass spectrometry (LC–MS/MS). It was found that the intravenously injected ATCA concentration quickly decreased to half within 2.5h in the rat system. However, after 2.5h, the concentration of ATCA in plasma stayed constant at least 5 folds above the endogenous ATCA level for more then 48h. This finding can be used for evaluating ATCA's diagnostic and forensic value as a biomarker for cyanide exposure.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2012.01.024