Heat-Transfer Resistance at Solid–Liquid Interfaces: A Tool for the Detection of Single-Nucleotide Polymorphisms in DNA

In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer...

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Bibliographic Details
Published in:ACS nano 2012-03, Vol.6 (3), p.2712-2721
Main Authors: van Grinsven, Bart, Vanden Bon, Natalie, Strauven, Hannelore, Grieten, Lars, Murib, Mohammed, Jiménez Monroy, Kathia L, Janssens, Stoffel D, Haenen, Ken, Schöning, Michael J, Vermeeren, Veronique, Ameloot, Marcel, Michiels, Luc, Thoelen, Ronald, De Ceuninck, Ward, Wagner, Patrick
Format: Article
Language:English
Subjects:
Base Sequence
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Brushes
Denaturation
Deoxyribonucleic acid
DNA - chemistry
DNA - genetics
DNA, Single-Stranded - chemistry
DNA, Single-Stranded - genetics
Electrodes
Fluorescence
Heat transfer
Hot Temperature
Mathematical models
Melting
Nucleic Acid Conformation
Nucleic Acid Denaturation
Oligonucleotide Array Sequence Analysis
Phenylalanine Hydroxylase - genetics
Polymorphism
Polymorphism, Single Nucleotide
Surface Properties
Switching
Transition Temperature
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Summary:In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn300147e