New insight into the molecular characterization of isoniazid and rifampicin resistant Mycobacterium tuberculosis strains from Saudi Arabia

• Published in: Infection, genetics and evolution genetics and evolution, 2012-04, Vol.12 (3), p.549-556
• Main Authors: Varghese, Bright, Hillemann, Andreas, Wijayanti, Dian Rachma, Shoukri, Mohammed, Al-rabiah, Fahad, Al-Omari, Ruba, Al-Hajoj, Sahal
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacterial diseases; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Catalase - genetics; Catalase - metabolism; Cluster Analysis; DNA, Bacterial - genetics; DNA, Bacterial - metabolism; DNA-Directed RNA Polymerases; Drug resistance; Drug Resistance, Multiple, Bacterial; Epidemiology. Vaccinations; Female; General aspects; Genetic Variation; genotyping; Human bacterial diseases; Humans; Infectious diseases; Isoniazid; Isoniazid - pharmacology; Male; Medical sciences; Microbial Sensitivity Tests; Middle Aged; multiple drug resistance; Mutation; Mycobacterium tuberculosis; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Odds Ratio; Pharmacology. Drug treatments; Rifampicin; Rifampin - pharmacology; Saudi Arabia; Saudi Arabia - epidemiology; Tuberculosis; Tuberculosis - diagnosis; Tuberculosis - epidemiology; Tuberculosis - microbiology; Tuberculosis and atypical mycobacterial infections; Young Adult
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2012.01.024

► Genetic variations and mutations in drug resistant Mycobacterium tuberculosis. ► rpoB codon 531 and katG codon 315 mutations are more prevalent. ► Significant association of treatment history and drug resistance. ► Association of multi drug resistance with Beijing strains. ► High cluster rate of multi drug resistant isolates particularly Beijing strains. Data on the genetic variation of isolates of Mycobacterium tuberculosis and spectrum of mutations determining resistance to principal anti-tuberculosis drugs isoniazid (INH) and rifampicin (RIF) have not yet been studied in Saudi Arabia. One hundred and fifty-one clinical isolates of M. tuberculosis from different regions in the country showing resistance to RIF and INH were subjected to drug susceptibility testing, characterization of mutations conferring drug resistance and genotyping. Phenotypically 17 (11.3%) isolates were resistance to RIF, 75 (49.6%) were resistant to INH and 59 (39.1%) were resistant to both RIF and INH, respectively. Sixteen (10.6%), 74 (49%) and 56 (37.1%) were determined as resistant to RIF, INH and to both by line probe assay. High frequency of rpoB 531 mutations (67.1%) in RIF resistant strains and katG 315 mutations (65.2%) in INH resistant strains were found. Mutations responsible for INH resistance, katG 315 (P value