Inheritance of male fertility restoration of the cytoplasmic-nuclear male-sterile line NJCMS1A of soybean [Glycine max (L) Merr.]

• Published in: Euphytica 2005-09, Vol.145 (1-2), p.25-32
• Main Authors: Bai, Y.N, Gai, J.Y
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; allelism; Biological and medical sciences; cytoplasmic male sterility; dominant genes; Fertility; Fundamental and applied biological sciences. Psychology; gene segregation; Genetics; Genetics and breeding of economic plants; Glycine max; Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia; inheritance (genetics); lines; Male sterility; Plant breeding: fundamental aspects and methodology; plant fertility; pollen germination; restorer lines; Segregation; Soybeans
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-005-8497-1

At present, no report on inheritance of male fertility restoration has been released, yet more than 10 cytoplasmic-nuclear male-sterile soybean lines as well as their maintainers and restorers have been developed. Based on our previous work, 25 restorers for the male-sterile line NJCMS1A were identified and the inheritance of male fertility restoration for these restorers was studied. The results showed that F^sub 1^s between NJCMS1A and its restorers were completely male-fertile. The numbers of fertile and sterile plants in the F^sub 2^ population of Cross I (NJCMS1A × N23601) and Cross II (NJCMS1A × N23683) corresponded to a segregation ratio of 15:1, and the numbers of non-segregation lines, 3:1 segregation lines and 15:1 segregation lines in F^sub 2:3^ of the same two crosses fitted a 7:4:4 genotypic segregation ratio. The testcross BC^sub 1^F^sub 1^s between the F^sub 1^s of the above two crosses and NJCMS1A, NJCMS1B showed a 3:1 segregation ratio. Accordingly, it was inferred that two pairs of duplicate dominant genes controlled the male fertility restoration of NJCMS1A in both crosses. Meanwhile, F^sub 2^ of other 23 crosses between NJCMS1A and its 23 restorers showed a fertility segregation ratio of 3:1 or 15:1. The F^sub 1^s of the five testcrosses between NJCMS1A and the F^sub 1^s of five crosses selected from the above 23 crosses showed that fertility segregation was 3:1 in BC^sub 1^F^sub 1^s between NJCMS1A and F^sub 1^s of the crosses of which fertility segregation fitted 15:1 in F^sub 2^ population, while fertility segregation in BC^sub 1^F^sub 1^s was 1:1 for those fertility segregation fitted 3:1 in F^sub 2^ population. Allelism tests showed that restore genes of all restorers in the experiment were allelic to two pairs of dominant genes. All results showed that some restorers bore one pair of dominant restore gene and the others bore two pairs of duplicate dominant gene. The mechanism of F^sub 1^ male sterility of the cross N8855 × N2899 was discussed.[PUBLICATION ABSTRACT]