Detection of quantitative trait locus for leaffolder (Cnaphalocrocis medinalis (Guenée)) resistance in rice on linkage group 1 based on damage score and flag leaf width

• Published in: Euphytica 2007-09, Vol.157 (1-2), p.35-43
• Main Authors: Selvaraju, K, Shanmugasundaram, P, Mohankumar, S, Asaithambi, M, Balasaraswathi, R
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; chromosome mapping; Cnaphalocrocis medinalis; correlated traits; Correlation analysis; Fundamental and applied biological sciences. Psychology; Gene mapping; genetic markers; genetic resistance; Genetics; Genetics and breeding of economic plants; Genomics; inbred lines; insect pests; leafrollers; leaves; linkage groups; loci; microsatellite repeats; molecular sequence data; Oryza sativa; pest resistance; phenotype; phenotypic correlation; plant damage; plant pests; quantitative trait loci; rice; Varietal selection. Specialized plant breeding, plant breeding aims; width
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-007-9394-6

Rice leaffolder (RLF) (Cnaphalocrocis medinalis (Guenée) is a destructive and widespread insect pest throughout the rice growing regions in Asia. The genetics of resistance to RLF in rice is very complex and not thoroughly explored. The present study was conducted to detect the quantitative trait loci (QTL) associated with RLF resistance involving 176 recombinant inbred lines (RILs) of F₈ generation derived from a cross between IR36, a leaffolder susceptible variety and TNAULFR831311, a moderately resistant indica rice culture. Simple sequence repeat (SSR) markers were used to construct specific linkage groups of rice. All the RILs were screened to assess their level of resistance to RLF by measuring the leaf area damaged. Besides this, the length and width of the flag leaf of each RIL were measured since these two parameters were considered as correlated traits to the RLF resistance in rice. All the above parameters observed across the RILs showed quantitative variation. Correlation analysis revealed that damage score based on greenhouse screening was positively correlated with length and width of the flag leaf. Out of 364 SSR markers analysed, 90 were polymorphic between the parents. Multi-point analysis carried out on segregating 69 SSR marker loci linkage group wise resulted in construction of linkage map with eleven groups of 42 SSR markers. Through single marker analysis, 19 SSR markers were found to have putative association with the three phenotypic traits studied. Of these markers, RM472 was identified as a locus having major effect on RLF resistance trait based on length of the flag leaf. Interval mapping detected two QTLs on linkage group 1. Among these QTLs, the QTL flanked by RM576-RM3412 were found to be associated with width of the flag leaf and RLF resistance. The putative SSR markers associated with leaffolder resistance identified in the present study may be one of the loci contributing resistance to RLF in rice.