Standard quantitative assays for apoptosis

The term apoptosis refers to a peculiar morphology of cell death. It is of special interest because it can be triggered physiologically (and pathologically), and it is regulated by the actions of specific gene products. Therefore, it can in principle be activated and suppressed by medical interventi...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biotechnology 2001-11, Vol.19 (3), p.305-312
Main Authors: SQUIER, Margaret K. T, COHEN, J. John
Format: Article
Language:English
Subjects:
Apoptosis
Bacteria
Biological and medical sciences
Diverse techniques
Electrophoresis, Agar Gel
Fundamental and applied biological sciences. Psychology
Genes
Humans
Molecular and cellular biology
Studies
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The term apoptosis refers to a peculiar morphology of cell death. It is of special interest because it can be triggered physiologically (and pathologically), and it is regulated by the actions of specific gene products. Therefore, it can in principle be activated and suppressed by medical intervention. It thus is often important to determine whether cells are dying by apoptosis (or its less regulated counterpart, necrosis) and also to quantity the effect in a population of cells. Here the classic methods of apoptosis quantitation are described; they will be of particular use to those whose laboratories are set up for standard microscopical and biochemical techniques, who do apoptosis assays infrequently but wish them to be widely accepted and reproducible. A simple microscopic observation, using blue light illumination and a pair of fluorescent dyes, is recommended for most applications.
ISSN:1073-6085
1559-0305
1073-6085
DOI:10.1385/MB:19:3:305