Screening of variables in b-xylosidase recovery using cetyl trimethyl ammonium bromide reversed micelles

b-Xylosidase recovery by micelles using cetyl trimethyl ammonium bromide (CTAB) cationic surfactant was verified under different experimental conditions. A 2 super(5-1) fractional factorial design with center points was employed to verify the influence of the following factors on enzyme extraction:...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and biotechnology 2001-03, Vol.91-93 (1-9), p.719-728
Main Authors: Hasmann, Francislene-Andreia, Pessoa, Adalberto, Roberto, Ines-Conceicao
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:b-Xylosidase recovery by micelles using cetyl trimethyl ammonium bromide (CTAB) cationic surfactant was verified under different experimental conditions. A 2 super(5-1) fractional factorial design with center points was employed to verify the influence of the following factors on enzyme extraction: pH (x sub(1)), CTAB concentration (x sub(2)), electrical conductivity (x sub(3)), hexanol concentration (x sub(4)), and butanol concentration (x sub(5)). Statistical analysis of the results shows that of the fivevariables studied only hexanol and electrical conductivity did not have significant effects on the recovery of b-xylosidase. The other factors had significant effects in increasing order: (x sub(1))>(x sub(2))>(x sub(5)). The model predicts a recovery value of about 45%, which is similar to that obtained experimentally (43.5%).
ISSN:0273-2289
1559-0291
DOI:10.1385/ABAB:91-93:1-9:719