Fast rewarming after deep hypothermic circulatory arrest in rats impairs histologic outcome and increases NFB expression in the brain

Objective: Deep hypothermia is used as a neuroprotectant during cardiac surgery utilizing deep hypothermic circulatory arrest (DHCA), although the ideal rewarming strategy is not known yet. Some of the neuroprotective properties of hypothermia seem to be mediated by Nuclear Factor Kappa B (NFB) as a...

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Published in:Perfusion 2010-09, Vol.25 (5), p.349-354
Main Authors: Gordan, M Lucia, Kellermann, Kristine, Blobner, Manfred, Nollert, Georg, Kochs, Eberhard F, Jungwirth, Bettina
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container_issue 5
container_start_page 349
container_title Perfusion
container_volume 25
creator Gordan, M Lucia
Kellermann, Kristine
Blobner, Manfred
Nollert, Georg
Kochs, Eberhard F
Jungwirth, Bettina
description Objective: Deep hypothermia is used as a neuroprotectant during cardiac surgery utilizing deep hypothermic circulatory arrest (DHCA), although the ideal rewarming strategy is not known yet. Some of the neuroprotective properties of hypothermia seem to be mediated by Nuclear Factor Kappa B (NFB) as an important transcription factor. The current study was designed to investigate the effect of the rewarming rate on histologic outcome and cerebral NFB expression one day following DHCA in rats.Methods: With IRB approval, 20 rats were cannulated for cardiopulmonary bypass (CPB), cooled to a rectal temperature of 15-18C, subjected to 45min of DHCA and randomly assigned to either a slow (40 min) or a fast (20 min) rewarming protocol. At 24 hours post DHCA, the number of eosinophilic neurons was analyzed with hematoxylin and eosin (HE) staining, and NFB expression immunohistochemically. The two experimental groups were compared with untreated control rats.Results: HE staining showed more eosinophilic neurons in the motor cortex following fast rewarming (60 [15-388]) compared to slow rewarming (15 [10--21]) (p
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Some of the neuroprotective properties of hypothermia seem to be mediated by Nuclear Factor Kappa B (NFB) as an important transcription factor. The current study was designed to investigate the effect of the rewarming rate on histologic outcome and cerebral NFB expression one day following DHCA in rats.Methods: With IRB approval, 20 rats were cannulated for cardiopulmonary bypass (CPB), cooled to a rectal temperature of 15-18C, subjected to 45min of DHCA and randomly assigned to either a slow (40 min) or a fast (20 min) rewarming protocol. At 24 hours post DHCA, the number of eosinophilic neurons was analyzed with hematoxylin and eosin (HE) staining, and NFB expression immunohistochemically. The two experimental groups were compared with untreated control rats.Results: HE staining showed more eosinophilic neurons in the motor cortex following fast rewarming (60 [15-388]) compared to slow rewarming (15 [10--21]) (p&lt;0.05). Neuronal expression of NFB was increased in the fast rewarming group in both brain areas, the motor cortex (fast: 258 [135-393]; slow: 165 [80--212]; control: 73 [44-111]) as well as the hippocampus (fast: 243 [209-314]; slow: 202 [187-239]; control: 86 [68-108]) (p&lt;0.05). 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Neuronal expression of NFB was increased in the fast rewarming group in both brain areas, the motor cortex (fast: 258 [135-393]; slow: 165 [80--212]; control: 73 [44-111]) as well as the hippocampus (fast: 243 [209-314]; slow: 202 [187-239]; control: 86 [68-108]) (p&lt;0.05). 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title Fast rewarming after deep hypothermic circulatory arrest in rats impairs histologic outcome and increases NFB expression in the brain
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