Development of novel microscale system as immobilized enzyme bioreactor

This study involves a novel method for immobilized enzyme catalysis. The focus of the work was to design and construct a microscale bioreactor using microfabrication techniques traditionally employed within the semiconductor industry. Enzymes have been immobilized on the microreactor walls by incorp...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2002, Vol.98-100 (1-9), p.627-640
Main Authors: JONES, Francis, ZONGHUAN LU, ELMORE, Bill B
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bioreactors
Biotechnology
Catalysis
Continuous flow
Enzymes
Enzymes, Immobilized - metabolism
Equipment Design
Fabaceae - enzymology
Fundamental and applied biological sciences. Psychology
Glucan 1,4-alpha-Glucosidase - metabolism
Methods. Procedures. Technologies
Microchannels
Micrometers
Microreactors
Miniaturization - methods
Polydimethylsiloxane
Rhizopus - enzymology
Urease - metabolism
Various methods and equipments
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Description
Summary:This study involves a novel method for immobilized enzyme catalysis. The focus of the work was to design and construct a microscale bioreactor using microfabrication techniques traditionally employed within the semiconductor industry. Enzymes have been immobilized on the microreactor walls by incorporating them directly into the wall material. Fabricated microchannels have cross-sectional dimensions on the order of hundreds of micrometers, constructed using polydimethylsiloxane cast on silicon/SU-8 molds. The resulting ratio of high surface area to volume creates an efficient, continuous-flow reaction system. Transverse features also containing enzymes were molded directly into the channels.
ISSN:0273-2289
1559-0291
0273-2289
DOI:10.1385/ABAB:98-100:1-9:627