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Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm
The low density, detergent-insoluble membrane fraction (LD-DIM), where gangliosides are likely to be highly enriched, was prepared from sperm of two sea urchin species, Hemicentrotus pulcherrimus and Strongylocentrotus purpuratus. Immunoblotting showed the presence in the LD-DIM of two receptors for...
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| Published in: | Glycoconjugate journal 2000-03, Vol.17 (3 -4), p.205-214 |
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| container_end_page | 214 |
| container_issue | 3 -4 |
| container_start_page | 205 |
| container_title | Glycoconjugate journal |
| container_volume | 17 |
| creator | Ohta, K Sato, C Matsuda, T Toriyama, M Vacquier, V D Lennarz, W J Kitajima, K |
| description | The low density, detergent-insoluble membrane fraction (LD-DIM), where gangliosides are likely to be highly enriched, was prepared from sperm of two sea urchin species, Hemicentrotus pulcherrimus and Strongylocentrotus purpuratus. Immunoblotting showed the presence in the LD-DIM of two receptors for egg ligands, a glycosylphosphatidylinositol (GPI)-anchored protein, and four proteins which may be involved in signal transduction. Co-immunoprecipitation revealed that at least three proteins, the speract receptor, the 63kDa GPI-anchored protein and the alpha subunit of a heterotrimeric Gs protein, are localized in the LD-DIM. This suggests that the LD-DIM fraction may be a membrane microdomain for speract-speract receptor interaction, as well as the subsequent signal transduction pathway involved in induction of sperm respiration, motility and possibly the acrosome reaction. |
| doi_str_mv | 10.1023/a:1026589223811 |
| format | article |
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| ispartof | Glycoconjugate journal, 2000-03, Vol.17 (3 -4), p.205-214 |
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| subjects | Animals Blotting, Western Carbohydrate Sequence Cell Membrane - chemistry Cell Membrane - metabolism Detergents - chemistry Echinoidea Fluorescent Antibody Technique Gangliosides - immunology Gangliosides - metabolism Glycosphingolipids - chemistry Glycosphingolipids - metabolism GTP-Binding Protein alpha Subunits, Gs - metabolism Hemicentrotus pulcherrimus Male Molecular Sequence Data Precipitin Tests Proteins Receptors, Cell Surface - metabolism Sea Urchins Signal transduction Solubility Spermatozoa - chemistry Spermatozoa - metabolism Strongylocentrotus purpuratus |
| title | Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm |
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