Association of Src-family protein tyrosine kinases with sphingolipids in rat cerebellar granule cells differentiated in culture

Src family kinases play a relevant role in the development and differentiation of neuronal cells. They are abundant in sphingolipid-enriched membrane domains of many cell types, and these domains are hypothesized to function in bringing together molecules important to signal transduction. We studied...

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Bibliographic Details
Published in:Glycoconjugate journal 2000-03, Vol.17 (3 -4), p.223-232
Main Authors: Prinetti, A, Marano, N, Prioni, S, Chigorno, V, Mauri, L, Casellato, R, Tettamanti, G, Sonnino, S
Format: Article
Language:English
Subjects:
Animals
Carbohydrate Sequence
Cell Differentiation
Cell Membrane - metabolism
Cells, Cultured
Cerebellum - cytology
Cerebellum - metabolism
Gangliosides
Kinases
Lipids
Membrane Proteins - chemistry
Membrane Proteins - metabolism
Molecular Sequence Data
Precipitin Tests
Protein-Tyrosine Kinases - metabolism
Proteins
Rats
Rats, Sprague-Dawley
Signal transduction
Sphingolipids - metabolism
src-Family Kinases - metabolism
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Summary:Src family kinases play a relevant role in the development and differentiation of neuronal cells. They are abundant in sphingolipid-enriched membrane domains of many cell types, and these domains are hypothesized to function in bringing together molecules important to signal transduction. We studied the association of Src family tyrosine kinases and their negative regulatory kinase, Csk, with sphingolipids in sphingolipid-enriched domains of rat cerebellar granule cells differentiated in culture. We find that c-Src, Lyn and Csk are enriched in the sphingolipid-enriched fraction prepared from these cells. Coimmunoprecipitation experiments show that these and sphingolipids are part of the same domain. Cross-linking experiments with a photoactivable, radioactive GD1b derivative show that c-Src and Lyn, which are anchored to the membrane via a myristoyl chain, associate directly with GD1b. Csk, which is not inserted in the hydrophobic core of the membrane, is not photolabeled by this ganglioside. These results suggest that lipid-lipid, lipid-protein, and protein-protein interactions cooperate to maintain domain structure. We hypothesize that such interactions might play a role in the process of neuronal differentiation.
ISSN:0282-0080
1573-4986
DOI:10.1023/A:1026545424720