C-series polysialogangliosides are expressed on stellate neurons of adult human cerebellum

Until now 'c-series' polysialogangliosides were known to exist in human brain only during development and in some pathological conditions like Alzheimer's disease. Using thin-layer chromatography (TLC) and immunostaining with Q211 antibody (TLC-overlay technique) we have analysed ...

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Bibliographic Details
Published in:Glycoconjugate journal 1998-04, Vol.15 (4), p.423-426
Main Authors: Heffer-Lauc, M, Cacić, M, Serman, D
Format: Article
Language:English
Subjects:
Adult
Antibodies, Monoclonal
Brain
Cerebellum - cytology
Cerebellum - metabolism
Chromatography, Thin Layer
Gangliosides - chemistry
Gangliosides - immunology
Gangliosides - metabolism
Humans
Immunohistochemistry
Middle Aged
Neurons - classification
Neurons - cytology
Neurons - metabolism
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Summary:Until now 'c-series' polysialogangliosides were known to exist in human brain only during development and in some pathological conditions like Alzheimer's disease. Using thin-layer chromatography (TLC) and immunostaining with Q211 antibody (TLC-overlay technique) we have analysed 'c-series' gangliosides in four human cerebella (age 20, 47, 52 and 54 years). Four distinct ganglioside bands, most probably corresponding to GT1c, GQ1c, GP1c and GH1c were found to exist in the analysed brains, which is convincing demonstration of the existence of 'c-series' gangliosides in normal adult human brain. Immunohistochemical analysis was performed to locate polysialogangliosides in the analysed tissue. Q211 antibody was found to bind specifically to a single subpopulation of neurons in the molecular layer of adult cerebellum. According to their position and morphology these cells correspond to stellate neurons.
ISSN:0282-0080
1573-4986
DOI:10.1023/A:1006938221704