Differential gene expression during somatic embryogenesis in Coffea arabica L., revealed by RT-PCR differential display

Molecular and biochemical studies of somatic embryogenesis may help to shed light on the mechanisms governing this phenomenon. In this article, a differential display analysis approach was employed to investigate the changes taking place during the induction of somatic embryogenesis in leaf explants...

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Bibliographic Details
Published in:Molecular biotechnology 2002-05, Vol.21 (1), p.43-50
Main Authors: ROJAS-HERRERA, R, QUIROZ-FIGUEROA, F, MONFORTE-GONZALEZ, M, SANCHEZ-TEYER, L, LOYOLA-VARGAS, V. M
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Cloning, Molecular
Coffea - genetics
Coffea arabica
Coffee
Diverse techniques
Embryonic growth stage
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Molecular and cellular biology
Molecular Sequence Data
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Seeds - genetics
Sequence Homology, Nucleic Acid
Studies
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Summary:Molecular and biochemical studies of somatic embryogenesis may help to shed light on the mechanisms governing this phenomenon. In this article, a differential display analysis approach was employed to investigate the changes taking place during the induction of somatic embryogenesis in leaf explants and suspension cultures of coffee. Cloned fragments show homologies to several proteins reported in databases, but only one has previously been described as regulated during somatic embryogenesis. By a reverse dot blot modification, the expression pattern of such fragments was evaluated.
ISSN:1073-6085
1559-0305
1073-6085
DOI:10.1385/MB:21:1:043