Adaptation of Chinese Hamster Ovary Cells to High Potassium Ion-Containing Medium for Enhancement of Follicle-Stimulating Hormone Production

In this study, a suspension culture of recombinant Chinese hamster ovary (CHO) cells producing follicle-stimulating hormone (FSH) was used to investigate the effects of potassium ion (K+) on cell growth and FSH production. Cell growth was significantly suppressed at a K+ concentration higher than 60...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2007-08, Vol.12 (4), p.399-403
Main Authors: Yoon, S.K. (LG Life Sciences, Ltd., Daejeon, Republic of Korea), E-mail: skyoonb@lgls.co.kr, Jeong, M.H. (LG Life Sciences, Ltd., Daejeon, Republic of Korea)
Format: Article
Language:English
Subjects:
ADAPTACION
ADAPTATION
Adaptations
Cell growth
CHO cells
Culture media
Follicle-stimulating hormone
Growth rate
Media (culture)
Ovaries
Potassium
potassium ion
Proteins
Suspension culture
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, a suspension culture of recombinant Chinese hamster ovary (CHO) cells producing follicle-stimulating hormone (FSH) was used to investigate the effects of potassium ion (K+) on cell growth and FSH production. Cell growth was significantly suppressed at a K+ concentration higher than 60 mM, but specific FSH productivity (q∧FSH) was enhanced more than 2-fold compared to the value obtained at 4 mM K+. In an attempt to alleviate the cell growth suppression at a high K+ concentration, the cells were adapted at 60 mM K+ in a repeated batch mode.
ISSN:1226-8372
1976-3816
DOI:10.1007/bf02931062