Enantioselective hydrolysis of racemic styrene oxide by epoxide hydrolase ofRhodosporidium kratochvilovae SYU-08

Enantioselective hydrolysis for the production of chiral styrene oxide was investigated using the epoxide hydrolase activity of a newly isolatedRhodosporidium kratochvilovae SYU-08. The effects of reaction prameters--buffer type, pH, temperature, initial substrate concentrations, phenyl-1,2-ethanedi...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2003-10, Vol.8 (5), p.306-308
Main Authors: Lee, Ji Won, Lee, Eun Jung, Yoo, Seung Sik, Park, Sung Hoon, Kim, Hee Sook, Lee, Eun Yeol
Format: Article
Language:English
Subjects:
Enantiomers
Enzymes
Epoxide hydrolase
Hydrolysis
Optical activity
oxides
pH effects
Styrene
Styrene oxide
Styrenes
Substrates
Temperature effects
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Summary:Enantioselective hydrolysis for the production of chiral styrene oxide was investigated using the epoxide hydrolase activity of a newly isolatedRhodosporidium kratochvilovae SYU-08. The effects of reaction prameters--buffer type, pH, temperature, initial substrate concentrations, phenyl-1,2-ethanediol concentrations on hydrolysis rate, and enantioselectivity--were analyzed. Optically active (S)-styrene oxide with an enantiomeric excess higher than 99 % was obtained from its racemate with a yield of 38 % (theoretically 50% maximum yield) from an initial concentration of 80 mM.[PUBLICATION ABSTRACT]
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02949223