Production of recombinant hirudin in galactokinase-deficientSaccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding

The artificial gene coding for anticoagulant hirudin was placed under the control of theGAL10 promoter and expressed in the galactokinase-deficient strain (Δgal1) ofSaccharomyces cereivisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient productio...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2003-06, Vol.8 (3), p.183-186
Main Authors: Ramisetti, Srinivas, Kang, Hyun Ah, Rhee, Sang Ki, Kim, Chul Ho
Format: Article
Language:English
Subjects:
Anticoagulants
Batch culture
Carbon sources
Fed batch
Feeding
Fermentation
Galactokinase
Galactose
Glucose
Hirudin
Microbiology
Promoters
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Summary:The artificial gene coding for anticoagulant hirudin was placed under the control of theGAL10 promoter and expressed in the galactokinase-deficient strain (Δgal1) ofSaccharomyces cereivisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L.[PUBLICATION ABSTRACT]
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02935894