Concentration of 6-aminopenicillanic acid from penicillin bioconversion solution and its mother liquor by nanofiltration membrane

In this study, nanofiltration was applied to the concentration of the 6-aminopenicillinic acid (6-APA) from bioconverted penicillin solution and also to its mother liquor. The 6-APA in the solution was concentrated from 0.211 mol/L to 0.746 mol/L by nanofiltration. The final maximum concentration wa...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2001-06, Vol.6 (3), p.200-204
Main Authors: Cao, Xuejun, Wu, XingYan, Wu, Tong, Jin, Keming, Hur, Byung Ki
Format: Article
Language:English
Subjects:
Acids
Aminopenicillanic acid
Ammonium chloride
Bioconversion
Crystallization
Energy consumption
Liquor
Membranes
Nanofiltration
Nanotechnology
Penicillin
Potassium chloride
Recovery
Salts
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Summary:In this study, nanofiltration was applied to the concentration of the 6-aminopenicillinic acid (6-APA) from bioconverted penicillin solution and also to its mother liquor. The 6-APA in the solution was concentrated from 0.211 mol/L to 0.746 mol/L by nanofiltration. The final maximum concentration was 3.6 times higher than the initial concentration and the recovery yield was 97% to 99% of the original 6-APA. The concentrated solution was crystallized with the yields of 88.9-90.2% and the purity of the crystallized product was about 98%. The concentration of 6-APA in the mother liquor after crystallization was 0.014 mol/L and thus was concentrated 20-30 fold by nanofiltration and crystallization. The recovery of 6-APA was over 98%. The salts contained in the mother liquor, such as NH sub(4)Cl and KCl, could be removed by allowing them to permeate through the membrane.
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02932551