Regulation of cobalamin-like synthase gene expression through a zinc-responsive transcriptional element

Human CBWD3 protein, incorporating a C-terminal FLAG tag, was expressed transiently from a plasmid construct comprising the full open reading frame of the human CBDW3 gene in the vector pCMV6Entry (Origene) in chinese hamster ovary cells treated for 24 h with 3 or 100 μm Zn.

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Bibliographic Details
Published in:Proceedings of the Nutrition Society 2010, Vol.69 (OCE6), p.E467-E467, Article E467
Main Authors: Tyson, J., van der Hagen, E. A. E., Coneyworth, L. J., Bell, L. S., Hadfield, J. N., Ford, D.
Format: Article
Language:English
Subjects:
Bacteria
Gene expression
Homeostasis
Proteins
Regulation
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Description
Summary:Human CBWD3 protein, incorporating a C-terminal FLAG tag, was expressed transiently from a plasmid construct comprising the full open reading frame of the human CBDW3 gene in the vector pCMV6Entry (Origene) in chinese hamster ovary cells treated for 24 h with 3 or 100 μm Zn.
ISSN:0029-6651
1475-2719
DOI:10.1017/S0029665110003307