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Regulation of cobalamin-like synthase gene expression through a zinc-responsive transcriptional element
Human CBWD3 protein, incorporating a C-terminal FLAG tag, was expressed transiently from a plasmid construct comprising the full open reading frame of the human CBDW3 gene in the vector pCMV6Entry (Origene) in chinese hamster ovary cells treated for 24 h with 3 or 100 μm Zn.
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Published in: | Proceedings of the Nutrition Society 2010, Vol.69 (OCE6), p.E467-E467, Article E467 |
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container_end_page | E467 |
container_issue | OCE6 |
container_start_page | E467 |
container_title | Proceedings of the Nutrition Society |
container_volume | 69 |
creator | Tyson, J. van der Hagen, E. A. E. Coneyworth, L. J. Bell, L. S. Hadfield, J. N. Ford, D. |
description | Human CBWD3 protein, incorporating a C-terminal FLAG tag, was expressed transiently from a plasmid construct comprising the full open reading frame of the human CBDW3 gene in the vector pCMV6Entry (Origene) in chinese hamster ovary cells treated for 24 h with 3 or 100 μm Zn. |
doi_str_mv | 10.1017/S0029665110003307 |
format | article |
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ispartof | Proceedings of the Nutrition Society, 2010, Vol.69 (OCE6), p.E467-E467, Article E467 |
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language | eng |
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source | Cambridge Journals Online |
subjects | Bacteria Gene expression Homeostasis Proteins Regulation |
title | Regulation of cobalamin-like synthase gene expression through a zinc-responsive transcriptional element |
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