Prospects of in vitro production of thebaine in opium poppy ( Papaver somniferum L.)

A variant plant of opium poppy ( Papaver somniferum L.) having high thebaine was obtained from the dose of 10 kR (kilo rad) + 0.4% EMS during an extensive mutation breeding experiment. The M 2 seeds of variant plant were subjected to in vitro studies to investigate the prospects of thebaine producti...

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Bibliographic Details
Published in:Industrial crops and products 2010-11, Vol.32 (3), p.668-670
Main Authors: Chaterjee, Avijeet, Shukla, Sudhir, Mishra, Pratibha, Rastogi, Anu, Singh, S.P.
Format: Article
Language:English
Subjects:
Bioreactor
callus
chemical constituents of plants
cotyledons
Economy
extraction
leaves
micropropagation
Mutation
Papaver somniferum
plant breeding
production costs
seeds
stems
Thebaine
Tissue culture
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Summary:A variant plant of opium poppy ( Papaver somniferum L.) having high thebaine was obtained from the dose of 10 kR (kilo rad) + 0.4% EMS during an extensive mutation breeding experiment. The M 2 seeds of variant plant were subjected to in vitro studies to investigate the prospects of thebaine production through tissue culture. Alkaloid profile of variant showed higher thebaine in stem followed by leaf callus, stem callus and cotyledons. Such a high content of thebaine from tissue raised plant material opens a new vista for the extraction of thebaine at commercial level. A comparison was also made between in vivo and in vitro cost of production and extraction of thebaine.
ISSN:0926-6690
1872-633X
DOI:10.1016/j.indcrop.2010.04.007