Partitioning of recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) from plant cell suspension culture in PEG/sodium phosphate aqueous two-phase systems

Partitioning of human granulocyte-macrophage colony stimulating factor (hGM-CSF) was achieved in the aqueous two-phase systems (ATPSs) using a crude extract of transgenic tobacco cell suspension culture. This study examined the effects of polyethylene glycol (PEG) molecular weight and concentration...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2004-02, Vol.9 (1), p.12-16
Main Authors: Lee, Jae-Hwa, Loc, Nguyen-Hoang, Kwon, Tae-Ho, Yang, Moon-Sik
Format: Article
Language:English
Subjects:
ATP
Binary systems
Cell culture
Cell suspensions
Colonies
Colony-stimulating factor
Culture
Granulocyte-macrophage colony stimulating factor
Granulocytes
Leukocytes (granulocytic)
Macrophages
Molecular weight
Partitioning
Pharmaceutical industry
Phosphates
Plant cells
Polyethylene glycol
Room temperature
Sodium
Sodium phosphate
Suspension culture
Temperature effects
Tobacco
Transgenic plants
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Summary:Partitioning of human granulocyte-macrophage colony stimulating factor (hGM-CSF) was achieved in the aqueous two-phase systems (ATPSs) using a crude extract of transgenic tobacco cell suspension culture. This study examined the effects of polyethylene glycol (PEG) molecular weight and concentration and the effects of sodium phosphate concentration in different PEG/sodium phosphate systems on the partition coefficient,K. The best ATPS system was 5% PEG 8,000/1.6 M sodium phosphate after 2 h of incubation at room temperature. In this system, hGM-CSF was partitioned in the PEG-rich phase with a yield of 57.99% andK ^sub hGM-CSF^ of 8.12. In another system, 3% PEG 10,000/1.6 M sodium phosphate, hGM-CSF was also partitioned primarily in the top phase with a yield of 45.66% andK ^sub hGM-CSF^ of 7.64 after 2 h of incubation at room temperature.[PUBLICATION ABSTRACT]
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02949316