Antioxidative activity on human low density lipoprotein (LDL) oxidation by pentagalloic acid

The aim of this study was to investigate the efficiency of the pentagalloic acid compound in inhibiting the metal ions and cell lines that mediate in low density lipoprotein (LDL) oxidation. Pentagalloic acid prolonged the lag time preceeding the onset of conjugated diene formation. In chemically in...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2000-10, Vol.5 (5), p.366-371
Main Authors: Ryu, Beung Ho, Kim, Hee Sook, Moon, Yoon Hee, Yang, Seong Taek
Format: Article
Language:English
Subjects:
Acids
Antioxidants
Ascorbic acid
Atherosclerosis
Cell lines
Copper
Density
Electrophoretic mobility
Endothelial cells
Hydrogen peroxide
Ions
Lag time
Lipid peroxidation
Lipids
Lipoproteins
Lipoproteins (low density)
Low density lipoprotein
Macrophages
Malondialdehyde
Metal ions
Metals
Oxidation
Peroxyl radicals
Propane
Proteins
Thiobarbituric acid
Tocopherol
Vitamin E
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this study was to investigate the efficiency of the pentagalloic acid compound in inhibiting the metal ions and cell lines that mediate in low density lipoprotein (LDL) oxidation. Pentagalloic acid prolonged the lag time preceeding the onset of conjugated diene formation. In chemically induced LDL oxidation by Cu^sup 2+^ plus hydrogen peroxide or peroxyl radical generated by 2, 2'-azo-bis (2-amidino propane) hydrochloride (AAPH), pentagalloic acid inhibited LDL oxidation as monitored by measuring the thiobarbituric acid reactive substances (TBARS), malondialdehyde (MDA), and gel electrophoretic mobility. The physiological relevance of the antioxidative activity was validated at the cellular level where pentagalloic acid inhibited mouse macrophage J774 and endothelial cell-mediated LDL oxidation. When compared with several other antioxidants, pentagalloic acid showed a much higher ability than naturally occuring antioxidants, α-tocopherol and ascorbic acid, and the synthetic antioxidant, probucol.[PUBLICATION ABSTRACT]
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02942214