Development of a serial bioreactor system for direct ethanol production from starch usingAspergillus niger andSaccharomyces cerevisiae

Aspergillus niger hyphae were found to grow with unliquefied potato starch under aerobic conditions, but did not grow under anaerobic conditions. The raw culture ofA. niger catalyzed saccharification of potato starch to glucose, producing approximately 12 g glucose/L/day/ The extracellular enzyme ac...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2007-10, Vol.12 (5), p.566-573
Main Authors: Jeon, Bo Young, Kim, Soo Jin, Kim, Dae Hee, Na, Byung Kwan, Park, Doo Hyun, Tran, Hung Thuan, Zhang, Ruihong, Ahn, Dae Hee
Format: Article
Language:English
Subjects:
Aerobic conditions
Anaerobic conditions
Aspergillus niger
Bioreactors
Enzymatic activity
Enzymes
Ethanol
Extracellular enzymes
Fungi
Glucose
Hyphae
Lichens
Potatoes
Reactors
Solanum tuberosum
Starch
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Summary:Aspergillus niger hyphae were found to grow with unliquefied potato starch under aerobic conditions, but did not grow under anaerobic conditions. The raw culture ofA. niger catalyzed saccharification of potato starch to glucose, producing approximately 12 g glucose/L/day/ The extracellular enzyme activity was decreased in proportion to incubation time, and approximately 64% of initial activity was maintained after 3 days. At 50°C,A. niger hyphae growth stopped, while the extracellular enzyme activity peaked. On the basis of theA. niger growth property and enzyme activity, we designed a serial bioreactor system composed of four different reactors. Fungal hyphae were cultivated in reactor I at 30°C, uniquefied starch was saccharified to glycose by a fungal hyphae culture in reactors II and III at 50°C, and glucose was fermented to ethanol bySaccharomyces cerevisiae in reactor IV. The total glucose produced by fungal hyphae in reactor I and saccharification in reactor II was about 42 g/L/day. Ethanol production in reactor IV was approximately 22 g/L/day, which corresponds to about 79% of the theoretical maximum produced from 55 g starch/L/day.[PUBLICATION ABSTRACT]
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02931356