Maternal dietary loads of α-tocopherol depress protein kinase C signaling and synaptic plasticity in rat postnatal developing hippocampus and promote permanent deficits in adult offspring

• Published in: The Journal of nutritional biochemistry 2011, Vol.22 (1), p.60-70
• Main Authors: Betti, Michele, Ambrogini, Patrizia, Minelli, Andrea, Floridi, Alessandro, Lattanzi, Davide, Ciuffoli, Stefano, Bucherelli, Corrado, Prospero, Emilia, Frontini, Andrea, Santarelli, Lory, Baldi, Elisabetta, Benetti, Fernando, Galli, Francesco, Cuppini, Riccardo
• Format: Article
• Language: English
• Subjects: alpha-Tocopherol - administration & dosage; alpha-Tocopherol - metabolism; alpha-Tocopherol - toxicity; Animals; Biological and medical sciences; Brain development; Cell signaling; Dietary Supplements - toxicity; Down-Regulation; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; GAP-43 Protein - metabolism; Hippocampus - cytology; Hippocampus - growth & development; Hippocampus - metabolism; Intracellular Signaling Peptides and Proteins - metabolism; Lactation; Long-term potentiation; Male; Maternal Nutritional Physiological Phenomena; Membrane Proteins - metabolism; Memory Disorders - chemically induced; Morris water maze; Myristoylated Alanine-Rich C Kinase Substrate; Nerve Tissue Proteins - metabolism; Neuronal Plasticity; Neurons - cytology; Neurons - metabolism; Phosphorylation; Pregnancy; Prenatal Exposure Delayed Effects; Protein Kinase C - antagonists & inhibitors; Protein Kinase C - metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Signal Transduction; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vitamin E
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2009.11.014

Vitamin E (α-tocopherol) supplementation has been tested as prophylaxis against gestational disorders associated with oxidative damage. However, recent evidence showing that high maternal α-tocopherol intake can adversely affect offspring development raises concerns on the safety of vitamin E extradosages during pregnancy. Besides acting as an antioxidant, α-tocopherol depresses cell proliferation and modulates cell signaling through inhibiting protein kinase C (PKC), a kinase that is deeply involved in neural maturation and plasticity. Possible effects of α-tocopherol loads in the maturing brain, where PKC dysregulation is associated to developmental dysfunctions, are poorly known. Here, supranutritional doses of α-tocopherol were fed to pregnant and lactating dams to evaluate the effects on PKC signaling and morphofunctional maturation in offspring hippocampus. Results showed that maternal supplementation potentiates hippocampal α-tocopherol incorporation in offspring and leads to marked decrease of PKC phosphorylation throughout postnatal maturation, accompanied by reduced phosphorylation of growth-associated protein-43 and myristoylated alanine-rich C kinase substrate, two PKC substrates involved in neural development and plasticity. Although processes of neuronal maturation, synapse formation and targeting appeared unaffected, offspring of supplemented mothers displayed a marked reduction of long-term synaptic plasticity in juvenile hippocampus. Interestingly, this impairment persisted in adulthood, when a deficit in hippocampus-dependent, long-lasting spatial memory was also revealed. In conclusion, maternal supplementation with elevated doses of α-tocopherol can influence cell signaling and synaptic plasticity in developing hippocampus and promotes permanent adverse effects in adult offspring. The present results emphasize the need to evaluate the safety of supranutritional maternal intake of α-tocopherol in humans.