Enhanced lovastatin production by solid state fermentation ofMonascus ruber

The purpose of this study was to optimize the solid state cultivation ofMonascus ruber on sterile rice. A single-level-multiple-factor and a single-factor-multiple-level experimental design were employed to determine the optimal medium constituents and to optimize carbon and nitrogen source concentr...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2005-02, Vol.10 (1), p.78-84
Main Authors: Xu, Bao-Jun, Wang, Qi-Jun, Jia, Xiao-Qin, Sung, Chang-Keun
Format: Article
Language:English
Subjects:
Acetic acid
Carbon
Cholesterol
Chromatography
Design factors
Design of experiments
Experimental design
Fermentation
Glycerol
High performance liquid chromatography
Industrial production
Liquid chromatography
Lovastatin
Metabolites
Nitrogen sources
Optimization
Oryza sativa
Photodiodes
Powder
Rice
Sensors
Sodium nitrate
Sodium nitrates
Solid state
Solid state fermentation
Soybeans
Yeast
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Summary:The purpose of this study was to optimize the solid state cultivation ofMonascus ruber on sterile rice. A single-level-multiple-factor and a single-factor-multiple-level experimental design were employed to determine the optimal medium constituents and to optimize carbon and nitrogen source concentrations for lovastatin production. Simultaneous quantitative analyses of the β-hydroxyacid form and β-hydroxylactone for of lovastatin were performed by the high performance liquid chromatography (HPLC) method with a UV photodiode-array (PDA) detector. The total lovastatin yield (4[asymptotically =]6 mg/g, average of five repeats) was achieved by adding soybean powder, glycerol, sodium nitrate, and acetic acid at optimal composition of the medium increased by almost 2 times the yield observed prior to optimization. The experimental results also indicated that the β-hydroxylactone form of lovastatin (LFL) and the β-hydroxyacid form of lovastatin (AFL) simultaneously existed in solid state cultures ofMonascus ruber, while the latter was the dominant form in the middle-late stage of continued fermentation. These results indicate that optimized culture conditions can be used for industrial production of lovastatin to obtain high yields.[PUBLICATION ABSTRACT]
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02931187