Change of Insulin-like Growth Factor Gene Expression in Chinese Hamster Ovary Cells Cultured in Serum-free Media

Although the sera used in animal cell culture media provide the macromolecules, nutrients, hormones, and growth factors necessary to support cell growth, it could also be an obstacle to the production of recombinant proteins in animal cell culture systems used in many sectors of the biotechnology in...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2006-08, Vol.11 (4), p.319-324
Main Authors: Park, H.W. (Hanyang University, Seoul, Republic of Korea), An, S.K. (Konkuk University, Seoul, Republic of Korea), Choe, T.B. (Konkuk University, Seoul, Republic of Korea), E-mail: tbchoe@konkuk.ac.kr
Format: Article
Language:English
Subjects:
Biotechnology
Biotechnology industry
Cell culture
Cell growth
Chinese hamster ovary cells
Culture media
Data processing
Dietary supplements
DNA microarrays
Ethanolamine
EXPRESION GENICA
EXPRESSION DES GENES
GENE EXPRESSION
Genomes
Growth factors
Growth patterns
Hormones
IGF
Insulin
Insulin-like growth factors
Laboratories
Macromolecules
Media (culture)
Nutrients
Ovaries
Pharmaceuticals
Proteins
Selenium
serum-free media
Transferrin
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Summary:Although the sera used in animal cell culture media provide the macromolecules, nutrients, hormones, and growth factors necessary to support cell growth, it could also be an obstacle to the production of recombinant proteins in animal cell culture systems used in many sectors of the biotechnology industry. For this reason, many research groups, including our laboratory, have been trying to develop serum-free media (SFM) or serum-supplemented media (SSM) for special or multi-purpose cell lines.
ISSN:1226-8372
1976-3816
DOI:10.1007/bf03026247