Mouse spermatogonial stem cells obtain morphologic and functional characteristics of hematopoietic cells in vivo

BACKGROUND The aim of this study was to explore the plasticity and transdifferentiation potential of murine spermatogonial stem cells (SSCs) into hematopoietic cells. METHODS GFP+CD49f+H-2Kb− SSCs of male donor mice were isolated and injected into the bone marrow (BM) of Busulfan-treated GFP− female...

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Bibliographic Details
Published in:Human reproduction (Oxford) 2010-12, Vol.25 (12), p.3101-3109
Main Authors: Ning, Liang, Goossens, Ellen, Geens, Mieke, Van Saen, Dorien, Van Riet, Ivan, He, Dalin, Tournaye, Herman
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Transdifferentiation
Female
Gynecology. Andrology. Obstetrics
hematopoietic cells
Hematopoietic Stem Cells - physiology
Male
Medical sciences
Mice
pluripotent stem cells
Spermatogonia - cytology
spermatogonial stem cells
stem cell plasticity
Stem Cells - physiology
transdifferentiation
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Summary:BACKGROUND The aim of this study was to explore the plasticity and transdifferentiation potential of murine spermatogonial stem cells (SSCs) into hematopoietic cells. METHODS GFP+CD49f+H-2Kb− SSCs of male donor mice were isolated and injected into the bone marrow (BM) of Busulfan-treated GFP− female mice. Twelve weeks post-transplantation, the recipients were sacrificed and their BM, peripheral blood (PB) and spleen (SL) cells were collected and evaluated by phenotypical methods, i.e. fluorescence-activated cell sorting, immunohistochemistry and fluorescence in situ hybridization, and functional assays, i.e. colony-forming units assay and intra-BM transplantation. RESULTS Green fluorescent protein (GFP)- and Y chromosome-positive cells were observed in the BM, PB and SL of transplanted female mice. These cells presented phenotypical and functional characteristics of hematopoietic cells in vitro and in vivo. CONCLUSIONS Our results indicate that SSCs have the potential to transdifferentiate into hematopoietic cells in vivo.
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/deq269