High-resolution mapping of the S-locus in Turnera leads to the discovery of three genes tightly associated with the S-alleles

While the breeding system known as distyly has been used as a model system in genetics, and evolutionary biology for over a century, the genes determining this system remain unknown. To positionally clone genes determining distyly, a high-resolution map of the S-locus region of Turnera has been cons...

Full description

Saved in:
Bibliographic Details
Published in:Molecular genetics and genomics : MGG 2009-06, Vol.281 (6), p.673-685, Article 673
Main Authors: Labonne, Jonathan J. D, Goultiaeva, Alina, Shore, Joel S
Format: Article
Language:English
Subjects:
Acetyltransferases - genetics
Alleles
Amino Acid Sequence
Animal Genetics and Genomics
Biochemistry
Biomedical and Life Sciences
Chromosome Mapping
Cloning
Cloning, Molecular
Crosses, Genetic
DNA Primers - chemistry
DNA, Plant
Genes
Genes, Plant - genetics
Genetic Linkage
Genomics
Human Genetics
Life Sciences
Microbial Genetics and Genomics
Molecular Sequence Data
Original Paper
Phylogeny
Plant Genetics and Genomics
Proteins
Sequence Homology, Amino Acid
Sulfotransferases - genetics
Turnera
Turnera - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:While the breeding system known as distyly has been used as a model system in genetics, and evolutionary biology for over a century, the genes determining this system remain unknown. To positionally clone genes determining distyly, a high-resolution map of the S-locus region of Turnera has been constructed using segregation data from 2,013 backcross progeny. We discovered three putative genes tightly linked with the S-locus. An N-acetyltransferase (TkNACE) flanks the S-locus at 0.35 cM while a sulfotransferase (TkST1) and a non-LTR retroelement (TsRETRO) show complete linkage to the S-locus. An assay of population samples of six species revealed that TsRETRO, initially discovered in diploid Turnera subulata, is also associated with the S-allele in tetraploid T. subulata and diploid Turnera scabra. The sulfotransferase gene shows some level of differential expression in long versus short styles, indicating it might be involved in some aspect of distyly. The complete linkage of TkST1 and TsRETRO to the S-locus suggests that both genes may reside within, or in the immediate vicinity of the S-locus. Chromosome walking has been initiated using one of the genes discovered in the present study to identify the genes determining distyly.
ISSN:1617-4615
1617-4623
DOI:10.1007/s00438-009-0439-5