Peri-implant reconstruction using autologous periosteum-derived cells and guided bone regeneration

• Published in: Journal of clinical periodontology 2010-12, Vol.37 (12), p.1128-1136
• Main Authors: Ribeiro, Fernanda Vieira, Suaid, Fabricia Ferreira, Ruiz, Karina Gonzales Silverio, Rodrigues, Thaisângela Lopes, Carvalho, Marcelo Diniz, Nociti, Francisco Humberto, Sallum, Enilson Antonio, Casati, Márcio Zaffalon
• Format: Article
• Language: English
• Subjects: Alveolar Bone Loss - surgery; Alveolar Process - pathology; Animals; Biological and medical sciences; Bone; Bone growth; Bone Regeneration - physiology; Cell Differentiation - physiology; Cell Separation; Dental Implantation, Endosseous; Dental Implants; Dentistry; Dogs; e-PTFE membrane; Facial bones, jaws, teeth, parodontium: diseases, semeiology; guided bone regeneration; Guided Tissue Regeneration, Periodontal - methods; Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics; Mandible - pathology; Mandible - surgery; Maxillofacial surgery. Dental surgery. Orthodontics; Medical sciences; Membranes, Artificial; Microscopy, Electron, Scanning; Non tumoral diseases; Osseointegration - physiology; Osteogenesis - physiology; Otorhinolaryngology. Stomatology; periosteum; Periosteum - cytology; Periosteum - transplantation; Random Allocation; Reconstruction; Regeneration; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Tissue and Organ Harvesting; tissue engineering; Tissue Engineering - methods; Tissue Scaffolds; Transplantation, Autologous; Treatment Outcome
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/j.1600-051X.2010.01635.x

Ribeiro FV, Suaid FF, Ruiz KGS, Rodrigues TL, Carvalho MD, Nociti FH, Sallum EA, Casati MZ. Peri‐implant reconstruction using autologous periosteum‐derived cells and guided bone regeneration. J Clin Periodontol 2010; doi: 10.1111/j.1600‐051X.2010.01635.x. Aim: This investigation evaluated the bone healing in peri‐implant defects treated with periosteum‐derived cells (PCs) and guided bone regeneration (GBR). Material and Methods: PCs were harvested from six beagle dogs and characterized in vitro with regard to their osteogenic properties. The animals were subjected to teeth extraction in the mandible, and after 3 months of healing, implant sites were drilled, bone dehiscences were created and implants were placed. Dehiscences were randomly assigned to: PCs+GBR, GBR, PCs and non‐treated defects. After 3 months, the implants/adjacent tissues were processed. Bone‐to‐implant contact (BIC) bone fill (BF) within implant threads, and bone area (BA) in a zone lateral to the implant were obtained. Results: In vitro analyses confirmed the osteogenic potential of PCs. Histometrically, no statistically significant differences were observed among the PCs+GBR, GBR and PCs groups for both BF and BIC (p>0.05), whereas these groups showed statistically higher values, as compared with the non‐treated group (p