Additive effect of tetramethylpyrazine and deferoxamine in the treatment of spinal cord injury caused by aortic cross-clamping in rats

• Published in: Spinal cord 2011-02, Vol.49 (2), p.302-306
• Main Authors: Liang, Y, Yang, Q-H, Yu, X-D, Jiang, D-M
• Format: Article
• Language: English
• Subjects: 631/378/1687/1825; 631/92/436/2388; 692/700/565/1436/1437; Anatomy; Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Anti-Inflammatory Agents, Non-Steroidal - therapeutic use; Aorta, Abdominal - surgery; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cerebrospinal fluid. Meninges. Spinal cord; Deferoxamine - pharmacology; Deferoxamine - therapeutic use; Disease Models, Animal; Human Physiology; Injuries of the nervous system and the skull. Diseases due to physical agents; Male; Medical sciences; Nervous system (semeiology, syndromes); Neurochemistry; Neurology; Neuropsychology; Neurosciences; original-article; Pyrazines - pharmacology; Pyrazines - therapeutic use; Rats; Rats, Sprague-Dawley; Reperfusion Injury - drug therapy; Reperfusion Injury - etiology; Siderophores - pharmacology; Siderophores - therapeutic use; Spinal Cord Ischemia - drug therapy; Spinal Cord Ischemia - etiology; Traumas. Diseases due to physical agents
• ISSN: 1362-4393; 1476-5624
• DOI: 10.1038/sc.2010.113

Study design: Tetramethylpyrazine and deferoxamine are effective agents for nerve injury. Experiments in a Sprague–Dawley rat model of spinal cord injury were performed. Objectives: This study investigated the effects of tetramethylpyrazine and deferoxamine on neurological outcome and spinal cord hisotpathology after transient spinal cord ischemia in rats. Setting: Chongqing Medical University and Third Military Medical University, Chongqing, China. Methods: Spinal cord ischemia was induced in Sprague–Dawley rats by infrarenal aortic occlusion for 30 min followed by 72 h of reperfusion. Animals were divided into a sham group with a sham procedure; control group with aortic occlusion, but no pharmacological intervention applied; and treatment group with aortic occlusion, treated with tetramethylpyrazine and deferoxamine. After 72 h of reperfusion, neurological status was evaluated in the animals. A histopathological study of spinal cords was performed, and glutamate level and metabotropic glutamate receptor-1 (mGluR1) mRNA expression were determined. Results: All animals in the control group were completely paraplegic with 0% recovery. Tarlov criteria were significantly better in the animals treated with tetramethylpyrazine and deferoxamine than that in the control group ( P