Light-susceptibility of camptothecin production fromin vitro cultures ofCamptotheca acuminata Decne

Production of camptothecin (CPT) from callus cultures ofCamptotheca acuminata Decne was affected by light and culture conditions. Among the culture media tested, modified B5 medium containing 3% (w/v) sucrose, 2 mg/L 2,4-D, 2 times of MS medium vitamins, 500 mg/L casein hydrolysate, 250 mg/L myo-ino...

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Published in:Biotechnology and bioprocess engineering 2003-02, Vol.8 (1), p.32-36
Main Authors: Park, Young Goo, Kim, Mee Hee, Yang, Jae Kyung, Chung, Young Gwan, Choi, Myung Suk
Format: Article
Language:English
Subjects:
2,4-D
Activated carbon
Activated charcoal
Callus
Callus culture
Camptothecin
Casein
Cell culture
Cell growth
Charcoal
Culture media
Hydrolysates
Inositol
Inositols
Light effects
Media (culture)
Metabolites
Photoperiods
Physiology
Sucrose
Vitamins
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Summary:Production of camptothecin (CPT) from callus cultures ofCamptotheca acuminata Decne was affected by light and culture conditions. Among the culture media tested, modified B5 medium containing 3% (w/v) sucrose, 2 mg/L 2,4-D, 2 times of MS medium vitamins, 500 mg/L casein hydrolysate, 250 mg/L myo-inositol, 0.05% (w/v) activated charcoal, and 0.15% (w/v) gelite was used for callus induction. The highest cell growth and CPT production were obtained in dark and green light condition, respectively. Photoperiod has no effect on cell growth and CPT production. Both cell growth and CPT production were also influenced by combination ratio of red and blue light. Cell growth and CPT production were the highest in the ratio of red and blue light 9010.[PUBLICATION ABSTRACT]
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02932895