Projections of calcitonin gene-related peptide immunoreactive neurons in the vagal ganglia of the rat

• Published in: Journal of chemical neuroanatomy 2011, Vol.41 (1), p.55-62
• Main Authors: Hayakawa, Tetsu, Kuwahara-Otani, Sachi, Maeda, Seishi, Tanaka, Koichi, Seki, Makoto
• Format: Article
• Language: English
• Subjects: Animals; Calcitonin Gene-Related Peptide - metabolism; Immunohistochemistry; Immunohistochemistry - methods; Jugular ganglion; Male; Nociceptive neurons; Nociceptors - cytology; Nodose Ganglion - cytology; Nucleus tractus solitarii; Rats; Rats, Sprague-Dawley; Retrograde tracing; Sensory Receptor Cells - cytology; Solitary Nucleus - cytology; Vagal sensory neurons; Vagus nerve; Viscera - innervation
• ISSN: 0891-0618; 1873-6300
• DOI: 10.1016/j.jchemneu.2010.11.003

This paper clarified that the CGRP-ir fibers and terminals in the caudal solitary nucleus come from the jugular ganglion neurons, and the peripheral targets of the CGRP-ir neurons located in the jugular ganglion of the vagus nerve. We have studied the connections of calcitonin gene-related peptide immunoreactive (CGRP-ir) sensory neurons in the ganglia of the vagus nerve. Many CGRP-ir neurons were identified in the jugular ganglion located in the cranial cavity, while fewer CGRP-ir neurons were found in the nodose ganglion located at the level of the jugular foramen. Application of Fluorogold to the cut end of the cervical vagus nerve resulted in many Fluorogold-labeled neurons in both the jugular and the nodose ganglia. Application of Fluorogold to the cut end of the subdiaphragmatic vagus nerve resulted in Fluorogold-labeled neurons mostly in the nodose ganglion with only a few labeled neurons in the jugular ganglion. Injection of Fluorogold into the heart resulted in Fluorogold-labeled neurons in both the jugular and the nodose ganglia. Double labeling combining CGRP immunohistochemistry and Fluorogold retrograde tracing showed that in cases of both the application of Fluorogold to the cut end of the cervical vagus nerve and the injection of Fluorogold into the heart, about 40% of the Fluorogold-labeled neurons in the jugular ganglion expressed CGRP-like immunoreactivity. These results indicate that many CGRP-ir neurons in the jugular ganglion innervate the cervical and thoracic visceral organs, including the heart, but only a few CGRP-ir neurons project to the abdominal visceral organs.