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Metabolically assessed muscle fibre recruitment in brief isometric contractions at different intensities
This study investigated the recruitment of type I, IIA and IIAX fibres after seven isometric contractions at 40, 70 and 100% maximal voluntary knee extension torque (MVC, 1 s on/1 s off). Biopsies of the vastus lateralis muscle were collected from seven subjects at rest and immediately post-exercise...
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| Published in: | European journal of applied physiology 2004-08, Vol.92 (4-5), p.485-492 |
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| Main Authors: | , , , , , |
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| container_end_page | 492 |
| container_issue | 4-5 |
| container_start_page | 485 |
| container_title | European journal of applied physiology |
| container_volume | 92 |
| creator | Beltman, J G M de Haan, A Haan, H Gerrits, H L van Mechelen, W Sargeant, A J |
| description | This study investigated the recruitment of type I, IIA and IIAX fibres after seven isometric contractions at 40, 70 and 100% maximal voluntary knee extension torque (MVC, 1 s on/1 s off). Biopsies of the vastus lateralis muscle were collected from seven subjects at rest and immediately post-exercise. Fibre fragments were dissected from the freeze-dried samples and characterized as type I, IIA and IIAX using mATPase staining. Phosphocreatine (PCr) and creatine (Cr) content were measured in the remaining part of characterized fibres. A decline in the ratio of PCr to Cr (PCr/Cr) was used as an indication of activation. The mean peak torques were, respectively, 39 (2), 72 (2) and 87 (6)% MVC. Cumulative distributions of type I and IIA fibres were significantly shifted to lower PCr/Cr ratios at all intensities (Kolmogorov-Smirnov test, P |
| doi_str_mv | 10.1007/s00421-004-1105-6 |
| format | article |
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Biopsies of the vastus lateralis muscle were collected from seven subjects at rest and immediately post-exercise. Fibre fragments were dissected from the freeze-dried samples and characterized as type I, IIA and IIAX using mATPase staining. Phosphocreatine (PCr) and creatine (Cr) content were measured in the remaining part of characterized fibres. A decline in the ratio of PCr to Cr (PCr/Cr) was used as an indication of activation. The mean peak torques were, respectively, 39 (2), 72 (2) and 87 (6)% MVC. Cumulative distributions of type I and IIA fibres were significantly shifted to lower PCr/Cr ratios at all intensities (Kolmogorov-Smirnov test, P<0.05). The cumulative distribution of type IIAX fibres showed a significant leftward shift only at 87% MVC ( P<0.05). A hierarchical order of fibre activation with increasing intensity of exercise was found, with some indication of rate coding for type I and IIA fibres. Evidence for activation of type IIAX fibres was only found at 87% MVC.</description><identifier>ISSN: 1439-6319</identifier><identifier>EISSN: 1439-6327</identifier><identifier>DOI: 10.1007/s00421-004-1105-6</identifier><identifier>PMID: 15138833</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Adenosine Triphosphatases - physiology ; Adult ; Biopsy ; Creatine - physiology ; Electromyography ; Female ; Histocytochemistry ; Humans ; Isometric Contraction - physiology ; Leg - physiology ; Male ; Muscle Fibers, Skeletal - enzymology ; Muscle Fibers, Skeletal - metabolism ; Muscle Fibers, Skeletal - physiology ; Muscle, Skeletal - enzymology ; Muscle, Skeletal - physiology ; Phosphocreatine - physiology</subject><ispartof>European journal of applied physiology, 2004-08, Vol.92 (4-5), p.485-492</ispartof><rights>Springer-Verlag 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-8f7a078346f354a8370dab64add0076b50f7684250fb1b5dd038168d4df81de03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15138833$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beltman, J G M</creatorcontrib><creatorcontrib>de Haan, A</creatorcontrib><creatorcontrib>Haan, H</creatorcontrib><creatorcontrib>Gerrits, H L</creatorcontrib><creatorcontrib>van Mechelen, W</creatorcontrib><creatorcontrib>Sargeant, A J</creatorcontrib><title>Metabolically assessed muscle fibre recruitment in brief isometric contractions at different intensities</title><title>European journal of applied physiology</title><addtitle>Eur J Appl Physiol</addtitle><description>This study investigated the recruitment of type I, IIA and IIAX fibres after seven isometric contractions at 40, 70 and 100% maximal voluntary knee extension torque (MVC, 1 s on/1 s off). Biopsies of the vastus lateralis muscle were collected from seven subjects at rest and immediately post-exercise. Fibre fragments were dissected from the freeze-dried samples and characterized as type I, IIA and IIAX using mATPase staining. Phosphocreatine (PCr) and creatine (Cr) content were measured in the remaining part of characterized fibres. A decline in the ratio of PCr to Cr (PCr/Cr) was used as an indication of activation. The mean peak torques were, respectively, 39 (2), 72 (2) and 87 (6)% MVC. Cumulative distributions of type I and IIA fibres were significantly shifted to lower PCr/Cr ratios at all intensities (Kolmogorov-Smirnov test, P<0.05). The cumulative distribution of type IIAX fibres showed a significant leftward shift only at 87% MVC ( P<0.05). A hierarchical order of fibre activation with increasing intensity of exercise was found, with some indication of rate coding for type I and IIA fibres. 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| ispartof | European journal of applied physiology, 2004-08, Vol.92 (4-5), p.485-492 |
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| subjects | Adenosine Triphosphatases - physiology Adult Biopsy Creatine - physiology Electromyography Female Histocytochemistry Humans Isometric Contraction - physiology Leg - physiology Male Muscle Fibers, Skeletal - enzymology Muscle Fibers, Skeletal - metabolism Muscle Fibers, Skeletal - physiology Muscle, Skeletal - enzymology Muscle, Skeletal - physiology Phosphocreatine - physiology |
| title | Metabolically assessed muscle fibre recruitment in brief isometric contractions at different intensities |
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