Functional Properties of Mesenchymal Stem Cells Labeled with Magnetic Microparticles in Vitro and Analysis of Their Distribution after Transplantation

Mesenchymal stem cells enzymatically isolated from human placenta were labeled with magnetic fluorescent microparticles (d=0.96 μ). We showed that microparticles in high doses (>10 μl stock suspension per 1 ml culture medium) significantly inhibited cell proliferation in culture. In our work we d...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2010-12, Vol.150 (1), p.131-136
Main Authors: Konieva, A. A, Kholodenko, I. V, Shragina, O. A, Kholodenko, R. V, Burunova, V. V, Bibaeva, L. V, Yarygin, K. N, Yarygin, V. N
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - drug effects
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell culture
Cell Differentiation - drug effects
Cell proliferation
Cell Proliferation - drug effects
Cells, Cultured
differentiation
Female
Humans
Internal Medicine
Laboratory Medicine
magnetic fluorescent microparticles
Magnetics
Male
Mesenchymal Stem Cell Transplantation
mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - drug effects
Mesenchyme
microparticles
Nanoparticles - adverse effects
Nanoparticles - chemistry
Pathology
Placenta
Pregnancy
proliferation
Rats
Rats, Wistar
Stem cells
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
Transplantation
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Description
Summary:Mesenchymal stem cells enzymatically isolated from human placenta were labeled with magnetic fluorescent microparticles (d=0.96 μ). We showed that microparticles in high doses (>10 μl stock suspension per 1 ml culture medium) significantly inhibited cell proliferation in culture. In our work we determined the optimal concentration of particles not affecting physiological properties of mesenchymal stem cells: it does not change cell proliferation, does not induce apoptosis, and does not modulate their transdifferentiation into neuronal cells. In vivo experiments showed that the chosen particles allow easy visualization of transplanted cells ex vivo on sections of different tissues.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-010-1087-9