Posttraumatic Activity of Signal Pathways of Nuclear Factor κB in Mature Sensory Neurons

• Published in: Bulletin of experimental biology and medicine 2010-10, Vol.149 (4), p.474-478
• Main Authors: Gushchina, S. V., Magoulas, C. B., Yousaf, N., Richardson, P. M., Volkova, O. V.
• Format: Article
• Language: English
• Subjects: Animals; Axotomy; Biomedical and Life Sciences; Biomedicine; Cell Biology; Chemokine CCL2 - genetics; DNA; Female; Ganglia, Spinal - metabolism; Genes; Genetic transcription; I-kappa B Proteins - genetics; Internal Medicine; Laboratory Medicine; Male; Neurons; NF-kappa B - physiology; NF-KappaB Inhibitor alpha; Pathology; Rats; Rats, Wistar; RNA; RNA, Messenger - metabolism; Sciatic Nerve - injuries; Sciatic Nerve - physiology; Sensory Receptor Cells - drug effects; Sensory Receptor Cells - metabolism; Tumor necrosis factor; Tumor Necrosis Factor-alpha - pharmacology
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-010-0974-4

The aim of this study was to clear out whether injury to the peripheral nerve leads to activation of nuclear factor κB in mature spinal ganglia. Analysis of matrix RNA of nuclear factor κB-dependent genes (monocyte chemoattractant protein MCP-1 and inhibitor of nuclear factor κB IκBα) showed different levels of expression of these genes in the spinal ganglia in vivo after axotomy and in vitro after TNF-α stimulation. On the other hand, DNA-binding activity of nuclear factor κB increased in the spinal ganglia 6 h after axotomy and after 10-min incubation of sensory neuron culture with TNF-α. These data attest to possible involvement of nuclear factor κB in the posttraumatic regulation of gene transcription in spinal ganglion cells.