In Vivo Fate Mapping and Expression Analysis Reveals Molecular Hallmarks of Prospectively Isolated Adult Neural Stem Cells

Until now, limitations in the ability to enrich adult NSCs (aNSCs) have hampered meaningful analysis of these cells at the transcriptome level. Here we show via a split-Cre technology that coincident activity of the hGFAP and prominin1 promoters is a hallmark of aNSCs in vivo. Sorting of cells from...

Full description

Saved in:
Bibliographic Details
Published in:Cell stem cell 2010-12, Vol.7 (6), p.744-758
Main Authors: Beckervordersandforth, Ruth, Tripathi, Pratibha, Ninkovic, Jovica, Bayam, Efil, Lepier, Alexandra, Stempfhuber, Barbara, Kirchhoff, Frank, Hirrlinger, Johannes, Haslinger, Anja, Lie, D. Chichung, Beckers, Johannes, Yoder, Bradley, Irmler, Martin, Götz, Magdalena
Format: Article
Language:English
Subjects:
Adult Stem Cells - cytology
Adult Stem Cells - metabolism
Animals
Biological and medical sciences
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Fundamental and applied biological sciences. Psychology
Glial Fibrillary Acidic Protein - genetics
Glial Fibrillary Acidic Protein - metabolism
Humans
Mice
Mice, Inbred C57BL
Molecular and cellular biology
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
Tumor Suppressor Proteins - genetics
Tumor Suppressor Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Until now, limitations in the ability to enrich adult NSCs (aNSCs) have hampered meaningful analysis of these cells at the transcriptome level. Here we show via a split-Cre technology that coincident activity of the hGFAP and prominin1 promoters is a hallmark of aNSCs in vivo. Sorting of cells from the adult mouse subependymal zone (SEZ) based on their expression of GFAP and prominin1 isolates all self-renewing, multipotent stem cells at high purity. Comparison of the transcriptome of these purified aNSCs to parenchymal nonneurogenic astrocytes and other SEZ cells reveals aNSC hallmarks, including neuronal lineage priming and the importance of cilia- and Ca-dependent signaling pathways. Inducible deletion of the ciliary protein IFT88 in aNSCs validates the role of ciliary function in aNSCs. Our work reveals candidate molecular regulators for unique features of aNSCs and facilitates future selective analysis of aNSCs in other functional contexts, such as aging and injury. ► Split-Cre fate mapping identifies GFAP/prominin1-expressing cells as aNSCs in vivo ► FACS sorting of GFAP/prominin1-expressing cells isolates aNSCs at high purity ► This approach distinguishes aNSCs from niche astrocytes and ependymal cells ► Transcriptome analysis revealed molecular characteristics of aNSCs
ISSN:1934-5909
1875-9777
DOI:10.1016/j.stem.2010.11.017