Mortality Impact of Bt Transgenic Maize Roots Expressing eCry3.1Ab, mCry3A, and eCry3.1Ab Plus mCry3A on Western Corn Rootworm Larvae in the Field

• Published in: Journal of economic entomology 2011-10, Vol.104 (5), p.1584-1591
• Main Authors: Hibbard, Bruce E., Frank, Daniel L., Kurtz, Ryan, Boudreau, Eric, Ellersieck, Mark R., Odhiambo, J. Frederick
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Animals; Bacillus thuringiensis; Bacillus thuringiensis - genetics; Bacterial Proteins - genetics; Bacterial Proteins - toxicity; Biological and medical sciences; Coleoptera; Control; corn; crystal proteins; Diabrotica virgifera virgifera; eclosion; eCry3.1Ab; Endotoxins - genetics; Endotoxins - toxicity; Feeding; Feeding Behavior; Female; FIELD AND FORAGE CROPS; Fundamental and applied biological sciences. Psychology; gender differences; Generalities; genetic background; Genetic engineering applications; Genetics and breeding of economic plants; Hemolysin Proteins - genetics; Hemolysin Proteins - toxicity; insecticidal proteins; Insecticides; Larva; larvae; Male; mCry3A; Missouri; Mortality; pest resistance; Phytopathology. Animal pests. Plant and forest protection; Plant breeding: fundamental aspects and methodology; Plant Roots - toxicity; plant-incorporated protectants; Plants, Genetically Modified - genetics; Protozoa. Invertebrates; Replication; resistance management; Roots; Survival; survival rate; transgenes; transgenic plants; Zea mays; Zea mays - genetics
• ISSN: 0022-0493; 1938-291X; 1938-291X; 0022-0493
• DOI: 10.1603/EC11186

Mortality of the western corn rootworm, Diabrotica virgifera virgifera LeConte, larvae due to feeding on maize, Zea mays L., expressing Bacillus thuringiensis Berliner (Bt) was evaluated in five Missouri sites in 2007, 2008, and 2009. Specifically, eCry3.1Ab (5307), mCry3A (MIR604), and eCry3.1Ab plus mCry3A proteins relative to survivorship on maize with the same genetic background without these genes (isoline maize) was evaluated. An average of 890.8 ± 152.3 beetles emerged from isoline plots, whereas average beetle emergence from 5307, MIR604, and 5307 × MIR604 was 1.9 ± 0.6,19.3 ± 6.3, and 0.8 ± 0.3, respectively, when averaged across 22 replications in five environments. Overall, 66, 50, 61, and 51% of beetles recovered from 5307, MIR604, 5307 × MIR604, and isoline maize, respectively, were female, and there was no significant difference between the number of male and female beetles that emerged from any of these treatments. Mortality due to 5307, MIR604, and 5307 × MIR604 was 99.79, 97.83, and 99.91%, respectively. There was an 8.0-d delay in time to 50% beetle emergence from 5307 compared with isoline maize, which was significantly later than to the other three maize lines. The average delay to 50% emergence from MIR604 and 5307 × MIR604 averaged 4.1 and 4.6 d, respectively later than 50% emergence from isoline maize. Female beetles had a significant delay in time to 50% emergence compared with male beetles from all treatments with the exception of 5307 × MIR604. Data are discussed in terms of insect resistance management in relation to other control measures for western corn rootworm.