Next-generation sequencing for understanding and accelerating crop domestication

Next generation Sequencing (NGS) provides a powerful tool for discovery of domestication genes in crop plants and their wild relatives. The accelerated domestication of new plant species as crops may be facilitated by this knowledge. Re-sequencing of domesticated genotypes can identify regions of lo...

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Bibliographic Details
Published in:Briefings in functional genomics 2012-01, Vol.11 (1), p.51-56
Main Author: Henry, Robert J.
Format: Article
Language:English
Subjects:
Biological variation
Crops
Crops, Agricultural - genetics
Data processing
Domestication
Genes
Genetic diversity
Genomes
Genotype & phenotype
Genotypes
Humans
Hybridization, Genetic
Intellectual Property
Oryza sativa
Polymerase chain reaction
Primers
proteomics
Quantitative Trait, Heritable
Sequence Analysis, DNA - methods
Species Specificity
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Summary:Next generation Sequencing (NGS) provides a powerful tool for discovery of domestication genes in crop plants and their wild relatives. The accelerated domestication of new plant species as crops may be facilitated by this knowledge. Re-sequencing of domesticated genotypes can identify regions of low diversity associated with domestication. Species-specific data can be obtained from related wild species by whole-genome shot-gun sequencing. This sequence data can be used to design species specific polymerase chain reaction (PCR) primers. Sequencing of the products of PCR amplification of target genes can be used to explore genetic variation in large numbers of genes and gene families. Novel allelic variation in close or distant relatives can be characterized by NGS. Examples of recent applications of NGS to capture of genetic diversity for crop improvement include rice, sugarcane and Eucalypts. Populations of large numbers of individuals can be screened rapidly. NGS supports the rapid domestication of new plant species and the efficient identification and capture of novel genetic variation from related species.
ISSN:2041-2649
1473-9550
2041-2647
2041-2657
DOI:10.1093/bfgp/elr032