Identification of a small-molecule inhibitor of dengue virus using a replicon system

Dengue virus (DENV) is a mosquito-borne human pathogen that causes a serious public-health threat in tropical and subtropical regions of the world. Neither a vaccine to prevent nor an effective therapeutic agent to treat DENV infection is currently available. We established a stable cell line harbor...

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Bibliographic Details
Published in:Archives of virology 2012-04, Vol.157 (4), p.681-688
Main Authors: Hsu, Yu-Chen, Chen, Nai-Chi, Chen, Po-Chiang, Wang, Chun-Chung, Cheng, Wei-Chieh, Wu, Huey-Nan
Format: Article
Language:English
Subjects:
Animals
Antiviral Agents - chemistry
Antiviral Agents - isolation & purification
Antiviral Agents - pharmacology
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cell Line
Dengue fever
Dengue virus
Dengue Virus - drug effects
Drug Evaluation, Preclinical - methods
Fundamental and applied biological sciences. Psychology
Genes, Reporter
Humans
Infectious Diseases
Luciferases - genetics
Luciferases - metabolism
Medical Microbiology
Microbiology
Miscellaneous
Molecular biology
Molecular Structure
Original Article
Pathogens
Plasmids
Proteins
Replicon - drug effects
Staining and Labeling
Vaccines
Virology
Virus Internalization - drug effects
Virus Replication - drug effects
Viruses
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Summary:Dengue virus (DENV) is a mosquito-borne human pathogen that causes a serious public-health threat in tropical and subtropical regions of the world. Neither a vaccine to prevent nor an effective therapeutic agent to treat DENV infection is currently available. We established a stable cell line harboring a luciferase-reporting DENV subgenomic replicon to screen for inhibitors of DENV. A total of 14,400 small-molecule (MW 
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-012-1224-z