Identification and characterization of gushing-active hydrophobins from Fusarium graminearum and related species

Fungal infection of barley and malt, particularly by the Fusarium species, is a direct cause of spontaneous overfoaming of beer, referred to as gushing. We have shown previously that small fungal proteins, hydrophobins, act as gushing‐inducing factors in beer. The aim of our present study was to iso...

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Bibliographic Details
Published in:Journal of basic microbiology 2012-04, Vol.52 (2), p.184-194
Main Authors: Sarlin, Tuija, Kivioja, Teemu, Kalkkinen, Nisse, Linder, Markus B., Nakari-Setälä, Tiina
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Beer - microbiology
Cloning, Molecular
DNA, Fungal - genetics
Fungal Proteins - genetics
Fungal Proteins - isolation & purification
Fungal Proteins - metabolism
Fusarium - genetics
Fusarium - metabolism
Fusarium graminearum
Genes, Fungal
Gibberella zeae
Gushing
Heterologous expression
Hordeum vulgare
Hydrophobin
Hypocrea jecorina
Markov Chains
Molecular Sequence Data
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Sequence Alignment
Sequence Analysis, DNA
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Summary:Fungal infection of barley and malt, particularly by the Fusarium species, is a direct cause of spontaneous overfoaming of beer, referred to as gushing. We have shown previously that small fungal proteins, hydrophobins, act as gushing‐inducing factors in beer. The aim of our present study was to isolate and characterize hydrophobins from a gushing‐active fungus, Fusarium graminearum (teleomorph Gibberella zeae) and related species. We generated profile hidden Markov models (profile HMMs) for the hydrophobin classes Ia, Ib and II from the multiple sequence alignments of their known members available in public domain databases. We searched the published Fusarium graminearum genome with the Markov models. The best matching sequences and the corresponding genes were isolated from F. graminearum and the related species F. culmorum and F. poae by PCR and characterized. One each of the putative F. graminearum and F. poae hydrophobin genes were expressed in the heterologous host Trichoderma reesei. The proteins corresponding to the genes were purified and identified as hydrophobins and named GzHYD5 and FpHYD5, respectively. Concentrations of 0.003 ppm of these hydrophobins were observed to induce vigorous beer gushing. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201100053