Effects of grain, fructose, and histidine on ruminal pH and fermentation products during an induced subacute acidosis protocol

• Published in: Journal of dairy science 2012-04, Vol.95 (4), p.1971-1982
• Main Authors: Golder, H.M., Celi, P., Rabiee, A.R., Heuer, C., Bramley, E., Miller, D.W., King, R., Lean, I.J.
• Format: Article
• Language: English
• Subjects: acetates; acidosis; Acidosis - etiology; Acidosis - metabolism; Acidosis - veterinary; alfalfa hay; ammonia; Ammonia - analysis; Animal productions; Animals; Biological and medical sciences; body weight; butyrates; Cattle; Cattle Diseases - etiology; Cattle Diseases - metabolism; Cereal and baking product industries; dairy cattle; diet; Diet - veterinary; dry matter intake; Edible Grain; Fatty Acids, Volatile - analysis; Female; fermentation; Fermentation - drug effects; Food industries; fructose; Fructose - administration & dosage; Fundamental and applied biological sciences. Psychology; grass silage; heifers; histamine; Histamine - blood; histidine; Histidine - administration & dosage; Holstein; Hydrogen-Ion Concentration; ingestion; lactate; Lactic Acid - analysis; Lactic Acid - blood; propionic acid; risk; rumen; Rumen - chemistry; Rumen - metabolism; rumen fermentation; Stomach Diseases - etiology; Stomach Diseases - veterinary; subacute ruminal acidosis; Terrestrial animal productions; triticale; Vertebrates; volatile fatty acids
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2011-4671

The effects of grain, fructose, and histidine on ruminal pH and fermentation products were studied in dairy cattle during an induced subacute acidosis protocol. Thirty Holstein heifers were randomly allocated to 5 treatment groups: (1) control (no grain); (2) grain [fed at a crushed triticale dry matter intake (DMI) of 1.2% of body weight (BW)]; (3) grain (0.8% of BW DMI)+fructose (0.4% of BW DMI); (4) grain (1.2% of BW DMI)+histidine (6g/head); and (5) grain (0.8% of BW DMI)+fructose (0.4% of BW DMI)+histidine (6g/head) in a partial factorial arrangement. Heifers were fed 1kg of grain daily with ad libitum access to ryegrass silage and alfalfa hay for 10d. Feed was withheld for 14h before challenge day, on which heifers were fed 200g of alfalfa hay and then the treatment diets immediately thereafter. Rumen samples were collected 5min after diet ingestion, 60min later, and at 3 subsequent 50-min intervals. Grain decreased ruminal pH and increased ammonia, total volatile fatty acid (VFA), acetate, butyrate, propionate, and valerate concentrations compared with controls. The addition of grain had no effect on ruminal d- and l-lactate concentrations. Fructose markedly decreased ruminal pH and markedly increased d- and l-lactate concentrations. Fructose increased total VFA and butyrate and decreased valerate concentrations. Although histidine did not have a marked effect on ruminal fermentation, increased concentrations of histamine were observed following feeding. This study demonstrates that the substitution of some grain for fructose can lower ruminal pH and increase VFA and lactate concentrations, warranting further investigation into the role of sugars on the risk of acidosis in dairy cattle.