Enzymatic production of xylooligosaccharides from alkali solubilized xylan of natural grass (Sehima nervosum)

► Identified natural grass (Sehima nervosum) as a raw material for xylooligosaccharides production. ► Able to maximize (98% of original xylan) xylan yield from natural grass by 12% NaOH coupled with steam application. ► Optimize the conditions for enzymatic production of xylooligosaccharides from th...

Full description

Saved in:
Bibliographic Details
Published in:Bioresource technology 2012-05, Vol.112, p.199-205
Main Authors: Samanta, A.K., Jayapal, Natasha, Kolte, A.P., Senani, S., Sridhar, Manpal, Suresh, K.P., Sampath, K.T.
Format: Article
Language:English
Subjects:
Alkalies - pharmacology
Disaccharides - metabolism
Endo-1,4-beta Xylanases - metabolism
Glucuronates - biosynthesis
Hydrogen-Ion Concentration - drug effects
Hydrolysis - drug effects
Hydroxides - pharmacology
Lignocellulosic biomass
Oligosaccharides - biosynthesis
Poaceae - chemistry
Potassium Compounds - pharmacology
RSM
Sehima nervosum
Sehima nervosum grass
Sodium Hydroxide - pharmacology
Solubility - drug effects
Spectroscopy, Fourier Transform Infrared
Temperature
Time Factors
Trisaccharides - metabolism
Xylan
Xylans - isolation & purification
Xylans - metabolism
Xylooligosaccharide
Xylose - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:► Identified natural grass (Sehima nervosum) as a raw material for xylooligosaccharides production. ► Able to maximize (98% of original xylan) xylan yield from natural grass by 12% NaOH coupled with steam application. ► Optimize the conditions for enzymatic production of xylooligosaccharides from the grass. ► Generates first report on xylooligosaccharides production from natural grass. In this study, a process for producing XOS from Sehima nervosum grass was developed. The grass contains 28.1% hemicellulose. NaOH and steam application yielded 98% of original xylan in contrast to 85% by KOH application. Hydrolysis of xylan with commercial xylanase caused breakdown into XOS comprising of xylobiose, xylotriose along with xylose. Response surface model (RSM) revealed highest xylobiose yield (11g/100g xylan) at pH 5.03, temperature 45.19°C, reaction time 10.11h with enzyme dose 17.41U. Similarly for maximizing xylotriose yield, ideal hydrolysis conditions were pH 5.11, temperature 40.33°C, reaction time 16.55h with enzyme dose 13.20U. A two step process encompassing xylan fractionation and enzymatic hydrolysis enabled XOS production from the S. nervosum grass.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.02.036