Ultrasound-assisted alkaline pretreatment of sugarcane bagasse for fermentable sugar production: Optimization through response surface methodology

Ultrasound-assisted alkaline pretreatment of sugarcane bagasse (SCB) for fermentable sugar production was carried out and the influence of particle size, liquid to solid ratio (LSR), NaOH concentration, temperature and sonication time on delignification and reducing sugar production was ascertained...

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Bibliographic Details
Published in:Bioresource technology 2012-05, Vol.112, p.293-299
Main Authors: Velmurugan, Rajendran, Muthukumar, Karuppan
Format: Article
Language:English
Subjects:
Analysis of Variance
Biotechnology - methods
Carbohydrate Metabolism - drug effects
Carbohydrates - biosynthesis
Cellulose - chemistry
Fermentation - drug effects
Lignin - metabolism
particle size
reducing sugars
Regression Analysis
response surface methodology
Saccharum - chemistry
Sodium Hydroxide - pharmacology
sugarcane bagasse
Ultrasonics - methods
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Summary:Ultrasound-assisted alkaline pretreatment of sugarcane bagasse (SCB) for fermentable sugar production was carried out and the influence of particle size, liquid to solid ratio (LSR), NaOH concentration, temperature and sonication time on delignification and reducing sugar production was ascertained with Placket–Burman design. The best combination of each significant factor was determined by a central composite design (CCD) and optimum pretreatment conditions for maximum reducing sugar yield (96.27%) were particle size of 0.27mm, LSR of 25ml/g, NaOH concentration of 2.89% (w/v), temperature of 70.15°C and pretreatment time of 47.42min. Under these conditions, 92.11% of theoretical reducing sugar yield was observed experimentally. The substantial reduction in pretreatment time and temperature with improved efficiency is the most attractive features of the ultrasound-assisted alkaline pretreatment.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.01.168