A tiered approach combining the short time exposure (STE) test and the bovine corneal opacity and permeability (BCOP) assay for predicting eye irritation potential of chemicals

For the evaluation of eye irritation, one in vitro alternative test may not completely replace the Draize test. Therefore, a tiered approach combining several in vitro assays, including cytotoxicity assays, is proposed in order to estimate the eye irritation potential of a wide range of chemical cla...

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Bibliographic Details
Published in:Journal of toxicological sciences 2012/04/01, Vol.37(2), pp.269-280
Main Authors: Hayashi, Kazuhiko, Mori, Taeko, Abo, Takayuki, Koike, Mirei, Takahashi, Yutaka, Sakaguchi, Hitoshi, Nishiyama, Naohiro
Format: Article
Language:English
Subjects:
Alternative method
Animals
BCOP
Cattle
Cell Line
Cornea
Cornea - drug effects
Cornea - pathology
Cytotoxicity
Data processing
Eye
Eye irritation
GHS
In Vitro Techniques
Irritants - toxicity
Irritation
Permeability
Rabbits
Reproducibility of Results
STE
Tiered approach
Toxicity Tests, Acute - methods
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Summary:For the evaluation of eye irritation, one in vitro alternative test may not completely replace the Draize test. Therefore, a tiered approach combining several in vitro assays, including cytotoxicity assays, is proposed in order to estimate the eye irritation potential of a wide range of chemical classes. The Short Time Exposure (STE) test, a relatively newer alternative eye irritation test, involves exposing Statens seruminstitut rabbit cornea (SIRC) cells for 5 min to two concentrations (5% and 0.05%) of test material. In the present study, we examined the predictive capacity of a tiered approach analyzing the results from the STE test and then the results of the bovine corneal opacity and permeability (BCOP) assay for assessing globally harmonized system (GHS) eye irritation rankings of various chemicals. The accuracy of predicting the GHS rankings was slightly improved when the tiered approach combination of STE test and BCOP assay was used compared to when the STE test irritation rank classification was used alone. Moreover, the under prediction rate was substantially improved when this tiered approach was used. From these results, the tiered approach of combining the data analysis of the STE test and BCOP assay might be a promising alternative eye irritation test strategy.
ISSN:0388-1350
1880-3989
DOI:10.2131/jts.37.269