Sensitivities of ciprofloxacin-resistant Mycobacterium tuberculosis clinical isolates to fluoroquinolones: role of mutant DNA gyrase subunits in drug resistance

Minimum inhibitory concentrations of sitafloxacin, gatifloxacin, moxifloxacin, sparfloxacin, levofloxacin and ciprofloxacin against 59 ciprofloxacin-resistant clinical isolates of Mycobacterium tuberculosis from Japan were determined. The isolates were most susceptible to sitafloxacin and gatifloxac...

Full description

Saved in:
Bibliographic Details
Published in:International journal of antimicrobial agents 2012-05, Vol.39 (5), p.435-439
Main Authors: Suzuki, Yasuhiko, Nakajima, Chie, Tamaru, Aki, Kim, Hyun, Matsuba, Takashi, Saito, Hajime
Format: Article
Language:English
Subjects:
amino acid sequences
Amino Acid Substitution
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antitubercular Agents - pharmacology
Biological and medical sciences
ciprofloxacin
Ciprofloxacin - pharmacology
DNA Gyrase - genetics
DNA topoisomerase (ATP-hydrolysing)
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
drug resistance
Drug Resistance, Bacterial
Fluoroquinolone resistance
Fluoroquinolones - pharmacology
gyrA
gyrB
Humans
Infectious Disease
Japan
Medical sciences
Microbial Sensitivity Tests
minimum inhibitory concentration
mutants
mutation
Mutation, Missense
Mutations
Mycobacterium tuberculosis
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - isolation & purification
nucleotide sequences
Pharmacology. Drug treatments
Protein Subunits - genetics
Sequence Analysis, DNA
sitafloxacin
sparfloxacin
Tuberculosis - microbiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Minimum inhibitory concentrations of sitafloxacin, gatifloxacin, moxifloxacin, sparfloxacin, levofloxacin and ciprofloxacin against 59 ciprofloxacin-resistant clinical isolates of Mycobacterium tuberculosis from Japan were determined. The isolates were most susceptible to sitafloxacin and gatifloxacin. To understand better the basis for drug resistance, nucleotide sequences encoding the gyrA and gyrB quinolone resistance-determining region were determined. Predicted amino acid sequences revealed distinct mutational patterns likely to be responsible for fluoroquinolone resistance. Double gyrA mutations as well as mutations in both gyrA and gyrB correlated with increased resistance to all fluoroquinolones.
ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2012.01.007